Determination of mivacurium in plasma by high-performance liquid chromatography

被引：7

作者：

WeindlmayrGoettel, M ^{[1
]}

Weberhofer, G ^{[1
]}

Gilly, H ^{[1
]}

Kress, HG ^{[1
]}

机构：

[1] L BOLTZMANN INST EXPT ANAESTHESIOL & RES INTENS C,VIENNA,AUSTRIA

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1996年 / 685卷 / 01期

关键词：

mivacurium;

D O I：

10.1016/0378-4347(96)00157-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An assay has been developed and validated for the routine monitoring of mivacurium in plasma. It consists of liquid-liquid extraction with dichloromethane and high-performance liquid chromatography with fluorometric detection (excitation and emission wavelengths 220 nm and 320 nm, respectively). A Spherisorb C-1 5 mu m column and a mobile phase containing acetonitrile, KH2PO4 and methanol are used. At a flow-rate of 1 ml/min, a concentration gradient is applied. The detection limit is approximately 1 ng/ml in plasma. For the separation of stereoisomeres, the Spherisorb SCX 10 mu m column and acetonitrile-Na2SO4 as a mobile phase can be used. The assay shows good linearity over the range 1-1000 ng/ml. The accuracy and precision allows the utilisation in clinical pharmacokinetic studies.

引用

页码：123 / 127

页数：5

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