Microchip-based liquid-liquid extraction for gas-chromatography analysis of amphetamine-type stimulants in urine

被引：49

作者：

Miyaguchi, Hajime

Tokeshi, Manabu

Kikutani, Yoshikuni

Hibara, Akihide

Inoue, Hiroyuki

Kitamori, Takehiko

机构：

[1] Natl Res Inst Police Sci, Kashiwa, Chiba 2770882, Japan

[2] Kanagawa Acad Sci & Technol, Micro Chem Grp, Takatsu Ku, Kawasaki, Kanagawa 2130012, Japan

[3] Univ Tokyo, Sch Engn, Dept Appl Chem, Tokyo 1138656, Japan

来源：

JOURNAL OF CHROMATOGRAPHY A | 2006年 / 1129卷 / 01期

关键词：

forensic toxicology; microfluidics; solvent extraction; gas chromatography; amphetamines; urine;

D O I：

10.1016/j.chroma.2006.06.107

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A microchip-based liquid-liquid extraction for the gas chromatography analysis of urine for amphetamine-type stimulants has been developed. Partially modified microchannels with the capillarity restricted modification (CARM) method were employed for stabilizing the interface consisting of 1-chlorobutane and alkalized urine. Reliability of the microchip-based extraction was evaluated with respect to linearity, trueness and precision. As a practical demonstration, methoxyphenamine hydrochloride (50 mg) was administered to three healthy volunteers, and the concentration of methoxyphenamine in their urine was determined by both methods for comparison. This study showed the potential of pressure-driven microfluidics to contribute to the rapid automation analysis in forensic toxicology. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：105 / 110

页数：6

共 14 条

[1] METABOLISM OF [C-14]METHAMPHETAMINE IN MAN, GUINEA-PIG AND RAT [J].