Drosophila Atlastin regulates the stability of muscle microtubules and is required for synapse development

被引:55
作者
Lee, Mihye [1 ,3 ]
Paik, Sang Kyoo [4 ]
Lee, Min-Jung [1 ]
Kim, Yoon-Jung [1 ]
Kim, Sungdae [1 ]
Nahm, Minyeop [1 ]
Oh, Soo-Jin [1 ,2 ]
Kim, Hyun-Man [1 ]
Yim, Jeongbin [3 ]
Lee, C. Justin [2 ]
Bae, Yong Chu [4 ]
Lee, Seungbok [1 ]
机构
[1] Seoul Natl Univ, Sch Dent, Dent Res Inst, Dept Cell & Dev Biol, Seoul 110740, South Korea
[2] Korea Inst Sci & Technol, Ctr Neural Sci, Seoul 136791, South Korea
[3] Seoul Natl Univ, Sch Biol Sci, Seoul 110740, South Korea
[4] Kyungpook Natl Univ, Sch Dent, Dept Oral Anat & Neurobiol, Taegu 700412, South Korea
关键词
Drosophila; Atl; Spastin; Synaptic growth; Neuromuscular junction; ER and Golgi morphogenesis; Microtubule stability; Hereditary spastic paraplegia; HEREDITARY SPASTIC PARAPLEGIA; TUMOR-SUPPRESSOR GENE; ENDOPLASMIC-RETICULUM; INTRACELLULAR-TRANSPORT; NEUROMUSCULAR-JUNCTION; PROTEIN SPASTIN; GOLGI-APPARATUS; GROWTH; SPG3A; AXON;
D O I
10.1016/j.ydbio.2009.03.019
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Hereditary spastic paraplegia (HSP) is an inherited neurological disorder characterized by progressive spasticity and weakness of the lower extremities. The most common early-onset form of HSP is caused by mutations in the human gene that encodes the dynamin-family GTPase Atlastin-1 (Atl-1). Recently, loss of the Drosophila ortholog of Atl-1 (Atl) has been found to induce locomotor impairments from the earliest adult stages, suggesting the developmental role of atlastin-subfamily GTPases. Here, we provide evidence that Ad is required for normal growth of muscles and synapses at the neuromuscular junction (NMJ). Ad protein is highly expressed in larval body-wall muscles. Loss-of-function mutations in the ad gene reduce the size of muscles and increase the number of synaptic boutons. Rescue of these defects is accomplished by muscular, but not neuronal expression of Ad. Loss of Ad also disrupts ER and Golgi morphogenesis in muscles and reduces the synaptic levels of the scaffold proteins Dlg and alpha-spectrin. We also provide evidence that Ad functions with the microtubule-severing protein Spastin to disassemble microtubules in muscles. Finally, we demonstrate that the microtubule-destabilizing drug vinblastine alleviates synapse and muscle defects in atl mutants. Together, our results suggest that Ad controls synapse development and ER and Golgi morphogenesis by regulating microtubule stability. (C) 2009 Elsevier Inc. All rights reserved.
引用
收藏
页码:250 / 262
页数:13
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