An avian sodium-hydrogen exchanger

被引:11
作者
Bhartur, SG
Bookstein, C
Musch, MW
Boxer, R
Chang, EB
Rao, MC
机构
[1] UNIV ILLINOIS,DEPT PHYSIOL & BIOPHYS MC901,CHICAGO,IL 60612
[2] UNIV CHICAGO,DEPT MED,CHICAGO,IL 60637
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-PHYSIOLOGY | 1997年 / 118卷 / 03期
关键词
chicken; intestine; epithelial; Na absorption; Na/H exchanger; brush border membrane; ion transport;
D O I
10.1016/S0300-9629(97)00219-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Intestinal sodium transporters, such as the Na+/H+ exchanger (NHE) are important for Na+ conservation in land birds. In mammals, at least five isoforms of the exchanger, NHEs 1-5, have been cloned, with NHE-1 occurring in epithelial basolateral and nonepithelial cell membranes and NHE-3 being restricted to epithelial apical/brush border membranes. We had demonstrated earlier that chicken intestinal brush border membranes possess NHE activity that functionally resembles mammalian NHE-3. In this study, we used mammalian NHE-1 and NHE-3 probes to examine if chicken enterocytes possess these transporters. Antisera against rat NHE-3 recognized a 97 kDa protein in chicken intestinal brush border membrane, while a NHE-3 cDNA probe failed to recognize any transcript. A NHE-1 antibody failed to recognize any protein in brush border or basolateral membrane, while a NHE-1 cDNA probe recognized a 3.9 kb transcript. Thus, there is more than one NHE isoform in chicken intestine, and our results suggest a novel avian NHE family. (C) 1997 Elsevier Science Inc.
引用
收藏
页码:883 / 889
页数:7
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