PCR detection and identification of oral streptococci in saliva samples using gtf genes

被引:124
作者
Hoshino, T
Kawaguchi, M
Shimizu, N
Hoshino, N
Ooshima, T
Fujiwara, T [1 ]
机构
[1] Nagasaki Univ, Grad Sch Biomed Sci, Div Pediat Dent, Nagasaki 8528588, Japan
[2] Osaka Univ, Grad Sch Dent, Dept Pediat Dent, Suita, Osaka 5650871, Japan
关键词
gtf gene; identification; oral streptococci; PCR;
D O I
10.1016/j.diagmicrobio.2003.10.002
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Oral streptococci are major constituents of dental plaque, and their prevalence is implicated in various pathologies. Therefore, accurate identification of oral streptococci would be valuable for studies of cariogenic plaque and for diagnostic use in infective endocarditis. Many oral streptococci possess glucosyltransferase enzymes that synthesize glucan, which is an obligate component of dental plaque. We established a rapid and precise method to identify oral streptococci by PCR using the species-specific region from the glucosyltransferase gene. With the species-specific primers, Streptococcus mutans, S. sobrinus, S. salivarius, S. sanguinis, S. oralis, and S. gordonii could be successfully distinguished. Further, we developed a simple method to extract the bacterial DNA from saliva. Using the resultant DNA as a template, the proposed PCR detection was performed. Their distribution was in accord with results of conventional biochemical tests. These findings indicate that the present PCR method is useful for the analysis of oral streptococci and can be successfully used in clinical applications to identify pathogenic bacteria associated with oral infectious disease and/or endocarditis. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:195 / 199
页数:5
相关论文
共 16 条
[1]   VIRULENCE FACTORS AMONG GRAM-POSITIVE BACTERIA IN EXPERIMENTAL ENDOCARDITIS [J].
BADDOUR, LM .
INFECTION AND IMMUNITY, 1994, 62 (06) :2143-2148
[2]   BACTEREMIA DUE TO VIRIDANS STREPTOCOCCUS IN NEUTROPENIC PATIENTS WITH CANCER - CLINICAL SPECTRUM AND RISK-FACTORS [J].
BOCHUD, PY ;
EGGIMAN, P ;
CALANDRA, T ;
VANMELLE, G ;
SAGHAFI, L ;
FRANCIOLI, P .
CLINICAL INFECTIOUS DISEASES, 1994, 18 (01) :25-31
[3]   Streptococcal meningitis in adult patients: Current epidemiology and clinical spectrum [J].
Cabellos, C ;
Viladrich, PF ;
Corredoira, J ;
Verdaguer, R ;
Ariza, J ;
Gudiol, F .
CLINICAL INFECTIOUS DISEASES, 1999, 28 (05) :1104-1108
[4]   OLIGONUCLEOTIDE PROBES FOR MUTANS STREPTOCOCCI [J].
CANGELOSI, GA ;
IVERSEN, JM ;
ZUO, Y ;
OSWALD, TK ;
LAMONT, RJ .
MOLECULAR AND CELLULAR PROBES, 1994, 8 (01) :73-80
[5]   IDENTITY OF VIRIDANS STREPTOCOCCI ISOLATED FROM CASES OF INFECTIVE ENDOCARDITIS [J].
DOUGLAS, CWI ;
HEATH, J ;
HAMPTON, KK ;
PRESTON, FE .
JOURNAL OF MEDICAL MICROBIOLOGY, 1993, 39 (03) :179-182
[6]   SIMPLE GENETIC METHOD TO IDENTIFY VIRIDANS GROUP STREPTOCOCCI BY COLORIMETRIC DOT HYBRIDIZATION AND FLUOROMETRIC HYBRIDIZATION IN MICRODILUTION WELLS [J].
EZAKI, T ;
HASHIMOTO, Y ;
TAKEUCHI, N ;
YAMAMOTO, H ;
LIU, SL ;
MIURA, H ;
MATSUI, K ;
YABUUCHI, E .
JOURNAL OF CLINICAL MICROBIOLOGY, 1988, 26 (09) :1708-1713
[7]  
Fujiwara T, 1998, FEMS MICROBIOL LETT, V161, P331, DOI 10.1111/j.1574-6968.1998.tb12965.x
[8]   Purification, characterization, and molecular analysis of the gene encoding glucosyltransferase from Streptococcus oralis [J].
Fujiwara, T ;
Hoshino, T ;
Ooshima, T ;
Sobue, S ;
Hamada, S .
INFECTION AND IMMUNITY, 2000, 68 (05) :2475-2483
[9]   BIOLOGY, IMMUNOLOGY, AND CARIOGENICITY OF STREPTOCOCCUS-MUTANS [J].
HAMADA, S ;
SLADE, HD .
MICROBIOLOGICAL REVIEWS, 1980, 44 (02) :331-384
[10]   Direct detection of Streptococcus mutans in human dental plaque by polymerase chain reaction [J].
Igarashi, T ;
Yamamoto, A ;
Goto, N .
ORAL MICROBIOLOGY AND IMMUNOLOGY, 1996, 11 (05) :294-298