Morphology of cell injury: An approach to the EDIT programme by the use of tobacco pollen tubes

被引：5

作者：

Kristen, U ^{[1
]}

Bischoff, N ^{[1
]}

Lisboa, S ^{[1
]}

Schirmer, E ^{[1
]}

Witt, S ^{[1
]}

Quader, H ^{[1
]}

机构：

[1] Univ Hamburg, Inst Allgemeine Bot, D-22609 Hamburg, Germany

来源：

ATLA-ALTERNATIVES TO LABORATORY ANIMALS | 2002年 / 30卷 / 03期

关键词：

cell injury; cell malformation; cytotoxicity; EDIT programme; MEIC programme; pollen germination; pollen tube growth; PTG test;

D O I：

10.1177/026119290203000310

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Tobacco pollen tubes were used as a standard in vitro system to investigate cell growth aberrations caused by some of the Multicentre Evaluation of in Vitro Cytotoxicity (MEIC) programme chemicals and other toxic compounds. Changes in cytoskeletal pattern were observed in the tube cells by using tubulin immunofluorescence and rhodamin-phalloidin fluorescence for the localisation of microtubules and actin filaments, respectively. Four different types of cell malformation were found: screw-like growth, isodiametric tip swelling, hook formation, and pollen grain enlargement. We suggest that these malformations resulted from an interference by the chemicals with the cytosolic calcium gradient which controls tip growth and the orientation of the pollen tube. The results may contribute to a general understanding of toxicity-based cell malformations.

引用

页码：323 / 329

页数：7

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