Development of Indica Basmati rice harboring two insecticidal genes for sustainable resistance against lepidopteran insects

被引：26

作者：

Riaz, N.

Husnain, T.

Fatima, T.

Makhdoom, R.

Bashir, K.

Masson, L.

Altosaar, I.

Riazuddin, S.

机构：

[1] Univ Punjab, Natl Ctr Excellence Mol Biol, Lahore, Pakistan

[2] Biotechnol Res Inst, Montreal, PQ H4P 2R2, Canada

[3] Univ Ottawa, Dept Biochem, Ottawa, ON K1N 6N5, Canada

来源：

SOUTH AFRICAN JOURNAL OF BOTANY | 2006年 / 72卷 / 02期

关键词：

Oryza sativa L; Bacillus thuringiensis; insect resistance; yellow stem borer; rice leaf folder; segregation analysis;

D O I：

10.1016/j.sajb.2005.07.005

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Rice variety Basmati 370 was transformed with a co-integrate vector pSM6 carrying cry1Ac and cry2A genes. The hygromycin resistance gene on a separate vector, pROB5 was used as a selectable marker. One to two week old scutellum derived calli were transformed with a biolistic gun. Transgenic status of different transformed lines was evaluated by molecular analysis (PCR, Dot blot, Southern blot, Western blot and ELISA) and insect feeding assays with rice leaf folder (RLF) and yellow stem borer (YSB). An average transformation efficiency of 1.98% was observed in a set of experiments. Plants from five different transgenic lines exhibited 100% mortality against rice leaf folder and segregated in Mendelian fashion. Insect bioassays revealed that there is no synergistic or antagonistic effect of cry1Ac and cry2A genes on the development of resistance against RFL and YSB. (c) 2005 SAAB. Published by Elsevier B.V. All rights reserved.

引用

页码：217 / 223

页数：7

共 34 条

[1] Effect of plant age, larval age, and fertilizer treatment on resistance of a cry1Ab-transformed aromatic rice to lepidopterous stem borers and foliage feeders [J].