Commitment of Annexin A2 in recruitment of microRNAs into extracellular vesicles

被引：70

作者：

Hagiwara, Keitaro ^{[1
,2
]}

Katsuda, Takeshi ^{[1
]}

Gailhouste, Luc ^{[1
]}

Kosaka, Nobuyoshi ^{[1
]}

Ochiya, Takahiro ^{[1
]}

机构：

[1] Natl Canc Ctr, Div Mol & Cellular Med, Chuo Ku, Tokyo 1040045, Japan

[2] Univ Occupat & Environm Hlth, Sch Med, Dept Biochem & Mol Pathophysiol, Fukuoka 8078555, Japan

来源：

FEBS LETTERS | 2015年 / 589卷 / 24期

基金：

日本学术振兴会;

关键词：

Proteomic; Annexin; Small interfering RNA; Cancer; MicroRNA; Extracellular vesicle; BINDING-SITE; RNA; COMPLEXES; MECHANISM; PROTEINS; EXOSOMES; HELICES;

D O I：

10.1016/j.febslet.2015.11.036

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Extracellular vesicles (EVs) contain microRNAs (miRNAs). However, the exact molecular mechanisms of the recruitment of miRNAs in EVs are not well characterized. Based on proteomic analysis, we identified that silencing of Annexin A2 (ANXA2) significantly decreased the amount of miRNAs in EVs. In addition, microarray analysis revealed that ANXA2 regulated the loading of miRNAs into EVs in a sequence independent manner. Lastly, immunoprecipitation analysis confirmed that ANXA2 could bind miRNAs in EVs in the presence of Ca2+. These observations demonstrate that ANXA2 plays an important role in the packaging process of miRNAs into EVs. (C) 2015 The Authors. Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：4071 / 4078

页数：8

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