The development of functional CD8 T cell memory after listeria monocytogenes infection is not dependent on CD40

被引:16
作者
Montfort, MJ
Bouwer, HGA
Wagner, CR
Hinrichs, DJ
机构
[1] Vet Affairs Med Ctr, Earle A Chiles Res Inst, Portland, OR 97239 USA
[2] Oregon Hlth & Sci Univ, Dept Mol Microbiol & Immunol, Portland, OR 97239 USA
[3] Oregon Hlth & Sci Univ, Dept Cardiol, Portland, OR 97239 USA
关键词
D O I
10.4049/jimmunol.173.6.4084
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The immunologic requirements for generating long-lived protective CD8 T cell memory remain unclear. Memory CD8 populations generated in the absence of CD4 Th cells reportedly have functional defects, and at least a subset of CD8 T cells transiently express CD40 after activation, suggesting that direct CD4-CD8 T cell interactions through CD40 may influence the magnitude and functional quality of memory CD8 populations. To ascertain the role of CD40 in such direct T cell interactions, we investigated CD8 T cell responses in CD40(-/-) mice after infection with Listeria monocytogenes, an intracellular bacterium that induces APC activation and thus priming of CD8 T cells independently of CD4 Th cell help through CD40. In this study we show that memory CD8 T cells generated in CD40-deficient mice show in vivo cytotoxicity and cytokine production equivalent to CD8 memory T cells from wild-type mice. Upon secondary Listeria infection, CD40(-/-) memory CD8 T cells expand to greater numbers than seen in wild-type mice. These results indicate that CD40 ligation on CD8 T cells, although reportedly a part of CD8 T cell memory development in an H-Y-directed response, is not needed for the development of functional memory CD8 T cell populations after Listeria infection.
引用
收藏
页码:4084 / 4090
页数:7
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