Role of Akt/protein kinase B in the activity of transcriptional coactivator p300

被引:56
作者
Chen, J
Halappanavar, SS
St-Germain, JR
Tsang, BK
Li, Q
机构
[1] Univ Ottawa, Ottawa Hlth Res Inst, Ottawa, ON K1Y 4E9, Canada
[2] Univ Ottawa, Dept Cellular & Mol Med, Ottawa, ON K1Y 4E9, Canada
[3] Univ Ottawa, Dept Med, Ottawa, ON K1Y 4E9, Canada
[4] Univ Ottawa, Dept Obstet & Gynecol, Ottawa, ON K1Y 4E9, Canada
关键词
Akt; protein kinase B; PI3K; p300; coactivator; protein degradation; proteasome; cellular signaling;
D O I
10.1007/s00018-004-4103-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 [生物化学与分子生物学]; 081704 [应用化学];
摘要
Akt/protein kinase B is a downstream target of the phosphatidylinositol 3-kinase (PI3K) pathway and plays a critical role in promotion of cell survival. The function of transcriptional coactivator p300 is required by many transcription factors to either activate or repress gene expression. Here, we show that induction of PI3K enhances the metabolic stability of endogenous p300 protein. On the other hand, repression of PI3K by LY294002 induces p300 degradation through the 26S proteasome pathway and impedes the transcriptional activity of the coactivator. In addition, Akt interacts with the coactivator and the activity of Akt is required to maintain the steady-state level of p300. Our study provides a new insight into the molecular mechanisms by which the critical concentration of p300 protein is regulated and suggests a role for Akt in control of various cellular activities through the transcriptional coactivator p300.
引用
收藏
页码:1675 / 1683
页数:9
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