A model of technical variation of microarray signals

被引：27

作者：

Chudin, E. ^{[1
]}

Kruglyak, S. ^{[1
]}

Baker, S. C. ^{[1
]}

Oeser, S. ^{[1
]}

Barker, D. ^{[1
]}

McDaniel, T. K. ^{[1
]}

机构：

[1] Illumina Inc, San Diego, CA 92131 USA

来源：

JOURNAL OF COMPUTATIONAL BIOLOGY | 2006年 / 13卷 / 04期

关键词：

microarray; quality metrics; model; fold change;

D O I：

10.1089/cmb.2006.13.996

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a mathematical model of signal from single-channel direct hybridization microarray platforms. The model establishes a linear relationship between microarray signals and their standard deviations from a minimum set of assumptions. We use the model to precisely define important microarray quality characteristics: resolved fold change and dynamic range. The definitions lead to closed form expressions relating these characteristics to physical parameters of the microarray experiment in the case when both specific and nonspecific binding of target to probe are governed by the Langmuir hybridization isotherm. The predictions of the model are in close agreement to data obtained from spike-in experiments. Given the generality of the model, the introduced definitions of dynamic range and resolved concentration fold-change can be used to conduct cross-platform comparisons and to guide improvement of the microarray platform.

引用

页码：996 / 1003

页数：8

共 9 条

[1]

Cedilnik A., 2004, METODOLOSKI ZVEZKI, V1, P99, DOI 10.51936/zweu3253

[2] Statistical tests for differential expression in cDNA microarray experiments [J].