Validated 5′ nuclease PCR assay for rapid identification of the genus Brucella

被引：38

作者：

Bogdanovich, T

Skurnik, M

Lübeck, PS

Ahrens, P

Hoorfar, J

机构：

[1] Danish Inst Food & Vet Res, DK-1790 Copenhagen, Denmark

[2] Univ Helsinki, Haartman Inst, Helsinki, Finland

[3] Univ Turku, Turku, Finland

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 05期

关键词：

D O I：

10.1128/JCM.42.5.2261-2263.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A real-time, genus-specific 5' nuclease PCR assay for amplification of a 322-bp fragment of the per gene was developed for rapid (<2 h) identification of Brucella spp. from agar plates. The assay, including an internal amplification control (116 bp), identified Brucella strains (n = 23) and did not detect non-Brucella strains (n = 174), indicating its usefulness, particularly for laboratories with stringent quality assurance programs.

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页码：2261 / 2263

页数：3

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