A novel arachidonate-preferring acyl-CoA synthetase is present in steroidogenic cells of the rat adrenal, ovary, and testis

被引：231

作者：

Kang, MJ ^{[1
]}

Fujino, T ^{[1
]}

Sasano, H ^{[1
]}

Minekura, H ^{[1
]}

Yabuki, N ^{[1
]}

Nagura, H ^{[1
]}

Iijima, H ^{[1
]}

Yamamoto, TT ^{[1
]}

机构：

[1] TOHOKU UNIV, SCH MED, DEPT PATHOL, SENDAI, MIYAGI 981, JAPAN

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1997年 / 94卷 / 07期

关键词：

cholesterol ester; fatty acid; high density lipoprotein; prostaglandins; leukotrienes;

D O I：

10.1073/pnas.94.7.2880

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We report herein the cDNA cloning of a novel rat acyl-CoA synthetase (ACS) that preferentially uses arachidonate and eicosapentaenoate. This newly identified ACS (designated ACS4) contains 670 amino acids and is 68% identical to rat ACS3, a previously characterized ACS that is highly expressed in brain, ACS I was overproduced in Escherichia coli and the resulting enzyme was purified to homogeneity. The purified enzyme utilizes arachidonate and eicosapentaenoate most preferentially among C-8-C-22 saturated fatty acids and C-14-C-22 unsaturated fatty acids, Kinetic analyses revealed that the enzyme has a high affinity for arachidonate and eicosapentaenoate and low affinity for palmitate. ACS4 transcripts are detectable in a wide range of tissues, with the highest level in adrenal gland, Immunoreactivity: to ACS4 was detected in the zona fasciculata and reticularis of adrenal gland, in the corpus luteum and stromal luteinized cells in ovary, and in the Leydig cells of testis.

引用

页码：2880 / 2884

页数：5

共 38 条

[1]

ANDERSEN JM, 1981, J BIOL CHEM, V256, P7362

[2]

[Anonymous], 1988, Antibodies: A Laboratory Manual

[3] THE UPTAKE OF THE APOPROTEIN AND CHOLESTERYL ESTER OF HIGH-DENSITY-LIPOPROTEINS BY THE PERFUSED-RAT-LIVER [J].

ARBEENY, CM ;

RIFICI, VA ;

EDER, HA .

BIOCHIMICA ET BIOPHYSICA ACTA, 1987, 917 (01) :9-17

[4]

BACHORIK PS, 1987, J BIOL CHEM, V262, P13636

[5] COMPARATIVE STUDIES OF RAT AND PIGEON LIVER FATTY ACID SYNTHETASES [J].

BURTON, DN ;

HAAVIK, AG ;

PORTER, JW .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1968, 126 (01) :141-&

[6] ROLE OF PHOSPHOLIPASES IN GENERATING LIPID 2ND MESSENGERS IN SIGNAL TRANSDUCTION [J].

DENNIS, EA ;

RHEE, SG ;

BILLAH, MM ;

HANNUN, YA .

FASEB JOURNAL, 1991, 5 (07) :2068-2077

[7] PROSTAGLANDIN SYNTHETASE-ACTIVITY IN BOVINE ADRENOCORTICAL-CELLS AND SUBCELLULAR PREPARATIONS - EFFECT OF ACTH [J].

DUPERRAY, A ;

CHAMBAZ, EM .

MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1980, 18 (03) :249-260

[8]

FITZPATRICK FA, 1988, PHARMACOL REV, V40, P229

[9]

FLEISHER LN, 1982, J BIOL CHEM, V257, P6653

[10] Molecular characterization and expression of rat acyl-CoA synthetase 3 [J].

Fujino, T ;

Kang, MJ ;

Suzuki, H ;

Iijima, H ;

Yamamoto, T .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (28) :16748-16752

← 1 2 3 4 →