Apisimin, a new serine-valine-rich peptide from honeybee (Apis mellifera L.) royal jelly:: purification and molecular characterization

被引：70

作者：

Bíliková, K

Hanes, J

Nordhoff, E

Saenger, W

Klaudiny, J

Simúth, J

机构：

[1] Slovak Acad Sci, Inst Chem, Lab Genet Engn, SK-84238 Bratislava, Slovakia

[2] Max Planck Inst Mol Genet, D-14195 Berlin, Germany

[3] Free Univ Berlin, Inst Chem Crystallog, D-14195 Berlin, Germany

来源：

FEBS LETTERS | 2002年 / 528卷 / 1-3期

关键词：

honeybee (Apis mellifera L.); royal jelly; apisimin; serine-valine-rich peptide; honeybee peptide;

D O I：

10.1016/S0014-5793(02)03272-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A peptide named apisimin was found in honeybee (Apis mellifera L.) royal jelly (RJ). N-terminal sequencing showed that this peptide corresponded to the sequence of a cDNA clone isolated from an expression cDNA library prepared from heads of nurse honeybees. No homology was found between the protein sequence of apisimin with a molecular mass of 5540.4 Da and sequences deposited in the Swiss-Prot database. The 54 amino acids of apisimin do not include Cys, Met, Pro, Arg, His, Tyr, and Trp residues. The peptide shows a well-defined secondary structure as observed by CD spectroscopy, and has the tendency to form oligomers. Isoelectrofocusing showed apisimin to be an acidic peptide. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：125 / 129

页数：5

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