Elicitation of silymarin in cell cultures of Silybum marianum: effect of subculture and repeated addition of methyl jasmonate

被引:16
作者
Angeles Sanchez-Sampedro, Maria [1 ]
Fernandez-Tarrago, Jorge [1 ]
Corchete, Purificacion [1 ]
机构
[1] Univ Salamanca, Fac Farm, Dept Plant Physiol, E-37007 Salamanca, Spain
关键词
Cell culture; Elicitation; Secondary metabolite; Silymarin; SUSPENSION-CULTURES; ELICITOR SIGNAL; ACCUMULATION; EVENTS;
D O I
10.1007/s10529-009-0043-0
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Production of silymarin and the effect of the elicitor, methyl jasmonate (MeJA), was monitored in cell cultures of Silybum marianum over 4 years. Silymarin concentrations gradually declined after prolonged subculture, making the success of elicitor strategy limited in long-term cultures. The continuous presence of MeJA in cultures for an extended period was necessary for induction of silymarin accumulation. A repeated elicitor strategy was not a good option for improving silymarin productivity in batch cultures. Removal of medium from elicited cultures and addition of fresh medium avoided the toxic effects of elicitor accumulation, allowing the system to respond to a repeated MeJA treatment without loss of productivity.
引用
收藏
页码:1633 / 1637
页数:5
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