Differential interaction of steroid hormone receptors with LXXLL motifs in SRC-1a depends on residues flanking the motif

被引：69

作者：

Needham, M

Raines, S

McPheat, J

Stacey, C

Ellston, J

Hoare, S

Parker, M

机构：

[1] AstraZeneca Pharmaceut, Macclesfield SK10 4TG, Cheshire, England

[2] Imperial Canc Res Fund, Mol Endocrinol Lab, London WC2A 3PX, England

来源：

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | 2000年 / 72卷 / 1-2期

关键词：

D O I：

10.1016/S0960-0760(00)00027-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Steroid hormones induce the transcriptional activity of their cognate receptors by recruiting a variety of cofactors. One of these, steroid receptor co-activator-1 (SRC-1) interacts with the ligand binding domains of a number of different receptors by means of LXXLL motifs. We have investigated the relative interaction of four such motifs in SR%-1a using a yeast two-hybrid assay. We demonstrate that ER alpha, ER beta and ER beta 2 preferentially interact with motif 2 while GR, AR, PPAR alpha and PPAR gamma preferentially interact with motif 4. We show that the interactions depend not only on the LXXLL motif itself but also on residues flanking the motif. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

收藏

页码：35 / 46

页数：12

相关论文

共 38 条

[1] AIB1, a steroid receptor coactivator amplified in breast and ovarian cancer [J].

Anzick, SL ;

Kononen, J ;

Walker, RL ;

Azorsa, DO ;

Tanner, MM ;

Guan, XY ;

Sauter, G ;

Kallioniemi, OP ;

Trent, JM ;

Meltzer, PS .

SCIENCE, 1997, 277 (5328) :965-968

[2] STEROID-HORMONE RECEPTORS - MANY ACTORS IN SEARCH OF A PLOT [J].

BEATO, M ;

HERRLICH, P ;

SCHUTZ, G .

CELL, 1995, 83 (06) :851-857

[3] Molecular basis of agonism and antagonism in the oestrogen receptor [J].

Brzozowski, AM ;

Pike, ACW ;

Dauter, Z ;

Hubbard, RE ;

Bonn, T ;

Engstrom, O ;

Ohman, L ;

Greene, GL ;

Gustafsson, JA ;

Carlquist, M .

NATURE, 1997, 389 (6652) :753-758

[4] INTERACTION OF PROTEINS WITH TRANSCRIPTIONALLY ACTIVE ESTROGEN-RECEPTORS [J].

CAVAILLES, V ;

DAUVOIS, S ;

DANIELIAN, PS ;

PARKER, MG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (21) :10009-10013

[5] Role of CBP/P300 in nuclear receptor signalling [J].

Chakravarti, D ;

LaMorte, VJ ;

Nelson, MC ;

Nakajima, T ;

Schulman, IG ;

Juguilon, H ;

Montminy, M ;

Evans, RM .

NATURE, 1996, 383 (6595) :99-103

[6] Nuclear receptor coactivator ACTR is a novel histone acetyltransferase and forms a multimeric activation complex with P/CAF and CBP/p300 [J].

Chen, HW ;

Lin, RJ ;

Schiltz, RL ;

Chakravarti, D ;

Nash, A ;

Nagy, L ;

Privalsky, ML ;

Nakatani, Y ;

Evans, RM .

CELL, 1997, 90 (03) :569-580

[7] ISOLATION AND CHARACTERIZATION OF A MAMMALIAN GENE ENCODING A HIGH-AFFINITY CAMP PHOSPHODIESTERASE [J].

COLICELLI, J ;

BIRCHMEIER, C ;

MICHAELI, T ;

ONEILL, K ;

RIGGS, M ;

WIGLER, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (10) :3599-3603

[8] IDENTIFICATION OF A CONSERVED REGION REQUIRED FOR HORMONE DEPENDENT TRANSCRIPTIONAL ACTIVATION BY STEROID-HORMONE RECEPTORS [J].

DANIELIAN, PS ;

WHITE, R ;

LEES, JA ;

PARKER, MG .

EMBO JOURNAL, 1992, 11 (03) :1025-1033

[9] Structure and specificity of nuclear receptor-coactivator interactions [J].

Darimont, BD ;

Wagner, RL ;

Apriletti, JW ;

Stallcup, MR ;

Kushner, PJ ;

Baxter, JD ;

Fletterick, RJ ;

Yamamoto, KR .

GENES & DEVELOPMENT, 1998, 12 (21) :3343-3356

[10] Nuclear receptor-binding sites of coactivators glucocorticoid receptor interacting protein 1 (GRIP1) and steroid receptor coactivator 1 (SRC-1): Multiple motifs with different binding specificities [J].

Ding, XF ;

Anderson, CM ;

Ma, H ;

Hong, H ;

Uht, RM ;

Kushner, PJ ;

Stallcup, MR .

MOLECULAR ENDOCRINOLOGY, 1998, 12 (02) :302-313

← 1 2 3 4 →