A transcript map of the chromosome 19q-arm glioma tumor suppressor region

被引:125
作者
Smith, JS
Tachibana, I
Pohl, U
Lee, HK
Thanarajasingam, U
Portier, BP
Ueki, K
Ramaswamy, S
Billings, SJ
Mohrenweiser, HW
Louis, DN
Jenkins, RB
机构
[1] Mayo Clin & Mayo Fdn, Sch Med, Dept Cytogenet, Rochester, MN 55905 USA
[2] Mayo Clin & Mayo Fdn, Dept Lab Med & Pathol, Div Lab Genet, Rochester, MN 55905 USA
[3] Massachusetts Gen Hosp, Mol Neurooncol Lab, Dept Pathol Neuropathol, Charlestown, MA 02129 USA
[4] Massachusetts Gen Hosp, Neurosurg Serv, Charlestown, MA 02129 USA
[5] Harvard Univ, Sch Med, Charlestown, MA 02139 USA
[6] Univ Calif Lawrence Livermore Natl Lab, Ctr Human Genome, Livermore, CA 94550 USA
关键词
D O I
10.1006/geno.1999.6101
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Allelic loss of the chromosome 19q arm is a frequent event in human diffuse gliomas, suggesting that it contains a tumor suppressor gene. Recent deletion mapping studies have broadly implicated a 1.6-Mb interval between D19S241E and D19S596, with a limited subset of tumors, suggesting that the region may be as narrow as 150 kb. Focusing on this smaller interval, we have used cDNA selection, exon amplification, and genomic sequencing to identify three novel transcripts (EHD2, GLTSCR1, and GLTSCR2) and to map two known genes (SEPW1 and CRX). A partial transcript map of 19 transcripts and two EST markers has been constructed for the 1.6-Mb interval D19S241E-D19S596. Ten of these transcripts, including the 5 mapped to the 150-kb deletion interval, have been examined for alterations in a panel of gliomas with allelic loss of 19q. Tumor-specific alterations have not been identified in the transcripts examined thus far. Collectively, these data should facilitate subsequent efforts to identify and characterize the remaining transcripts in the 1.6-Mb interval, (C) 2000 Academic Press.
引用
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页码:44 / 50
页数:7
相关论文
共 41 条
  • [1] ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999
  • [2] Roles for Nkx3.1 in prostate development and cancer
    Bhatia-Gaur, R
    Donjacour, AA
    Sciavolino, PJ
    Kim, M
    Desai, N
    Young, P
    Norton, CR
    Gridley, T
    Cardiff, RD
    Cunha, GR
    Abate-Shen, C
    Shen, MM
    [J]. GENES & DEVELOPMENT, 1999, 13 (08) : 966 - 977
  • [3] EXON AMPLIFICATION - A STRATEGY TO ISOLATE MAMMALIAN GENES BASED ON RNA SPLICING
    BUCKLER, AJ
    CHANG, DD
    GRAW, SL
    BROOK, JD
    HABER, DA
    SHARP, PA
    HOUSMAN, DE
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (09) : 4005 - 4009
  • [4] Specific genetic predictors of chemotherapeutic response and survival in patients with anaplastic oligodendrogliomas
    Cairncross, JG
    Ueki, K
    Zlatescu, MC
    Lisle, DK
    Finkelstein, DM
    Hammond, RR
    Silver, JS
    Stark, PC
    Macdonald, DR
    Ino, Y
    Ramsay, DA
    Louis, DN
    [J]. JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1998, 90 (19) : 1473 - 1479
  • [5] Church DM, 1999, METHOD ENZYMOL, V303, P83
  • [6] ISOLATION OF GENES FROM COMPLEX SOURCES OF MAMMALIAN GENOMIC DNA USING EXON AMPLIFICATION
    CHURCH, DM
    STOTLER, CJ
    RUTTER, JL
    MURRELL, JR
    TROFATTER, JA
    BUCKLER, AJ
    [J]. NATURE GENETICS, 1994, 6 (01) : 98 - 105
  • [7] Modeling stochastic gene expression: Implications for haploinsufficiency
    Cook, DL
    Gerber, LN
    Tapscott, SJ
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (26) : 15641 - 15646
  • [8] Pten is essential for embryonic development and tumour suppression
    Di Cristofano, A
    Pesce, B
    Cordon-Cardo, C
    Pandolfi, PP
    [J]. NATURE GENETICS, 1998, 19 (04) : 348 - 355
  • [9] Cone-rod dystrophy due to mutations in a novel photoreceptor-specific homeobox gene (CRX) essential for maintenance of the photoreceptor
    Freund, CL
    GregoryEvans, CY
    Furukawa, T
    Papaioannou, M
    Looser, J
    Ploder, L
    Bellingham, J
    Ng, D
    Herbrick, JAS
    Duncan, A
    Scherer, SW
    Tsui, LC
    LoutradisAnagnostou, A
    Jacobson, SG
    Cepko, CL
    Bhattacharya, SS
    McInnes, RR
    [J]. CELL, 1997, 91 (04) : 543 - 553
  • [10] FULTS D, 1990, CANCER RES, V50, P5784