Genotoxic effects of estradiol-17β on human lymphocyte chromosomes

被引：25

作者：

Ahmad, ME ^{[1
]}

Shadab, GGHA ^{[1
]}

Hoda, A ^{[1
]}

Afzal, M ^{[1
]}

机构：

[1] Aligarh Muslim Univ, Dept Zool, Genet Sect, Aligarh 202002, Uttar Pradesh, India

来源：

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS | 2000年 / 466卷 / 01期

关键词：

estradiol-17; beta; chromosomal aberration; sister chromatid exchange; lymphocyte;

D O I：

10.1016/S1383-5718(99)00230-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The cytogenetic effect of a hormonal steroid, estradiol-17 beta, was assessed in peripheral blood human lymphocyte culture. Sister chromatid exchanges (SCE) and chromosome aberrations (CA) were scored as genetic end points. Significant induction of CA was observed at 25 mu g/ml and 50 mu g/ml concentrations of estradiol-17 beta in the absence of microsomal activation. The drug was effective in all treatments in the presence of rat liver S-9 microsomal fraction (S-9 mix) and exhibited increased frequency of chromosomal aberrations. The drug was effective in increasing the SCE frequency which was found to be maximum at the dose of 50 mu g/ml concentration (i.e., 4.34 +/- 1.22) both with and without metabolic activation. It was found that estradiol-17 beta itself and possibly its metabolites are potent mutagens beyond a particular dose in human lymphocytes. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：109 / 115

页数：7

共 30 条

[1] MUTAGENICITY OF METHYL 2-BENZIMIDAZOLECARBAMATE, DIETHYLSTILBESTROL AND ESTRADIOL - STRUCTURAL CHROMOSOMAL-ABERRATIONS, SISTER-CHROMATID EXCHANGES, C-MITOSES, POLYPLOIDIES AND MICRONUCLEI [J].

BANDUHN, N ;

OBE, G .

MUTATION RESEARCH, 1985, 156 (03) :199-218

[2]

BLACKBURN GM, 1974, CANCER RES, V34, P2015

[3] SISTER CHROMATID EXCHANGE AS AN INDICATOR OF MUTAGENESIS [J].

CARRANO, AV ;

THOMPSON, LH ;

LINDL, PA ;

MINKLER, JL .

NATURE, 1978, 271 (5645) :551-553

[4] MUTAGENICITY ASSAYS OF ESTROGENIC HORMONES IN MAMMALIAN-CELLS [J].

DREVON, C ;

PICCOLI, C ;

MONTESANO, R .

MUTATION RESEARCH, 1981, 89 (01) :83-90

[5]

HAKEEM H, 1965, NATURWISSENSCHAFTEN, V53, P184

[6]

*IARC, 1979, IARC MON, V21

[7] THE ENHANCEMENT OF THE EFFECT OF AFLATOXIN-B1 BY METABOLIC-ACTIVATION WITH RAT-LIVER MICROSOMES ON HUMAN-LYMPHOCYTES ASSAYED WITH THE MICRONUCLEUS TEST [J].

ISKANDAR, O ;

VIJAYALAXMI .

MUTATION RESEARCH, 1981, 91 (01) :63-66

[8]

JOSHI NJ, 1983, INDIAN J EXP BIOL, V21, P591

[9]

KABRIATY S, 1984, MUTAT RES, V135, P181

[10] NON-MUTAGENICITY OF SOME SEX-HORMONES IN THE AMES SALMONELLA-MICROSOME MUTAGENICITY TEST [J].

LANG, R ;

REDMANN, U .

MUTATION RESEARCH, 1979, 67 (04) :361-365

← 1 2 3 →