A chloroplast derived trnH gene is expressed in the mitochondrial genome of gramineous plants

被引:11
作者
Kanno, A [1 ]
Nakazono, M [1 ]
Hirai, A [1 ]
Kameya, T [1 ]
机构
[1] UNIV TOKYO,GRAD SCH AGR & LIFE SCI,BUNKYO KU,TOKYO 113,JAPAN
关键词
chloroplast-derived trnH; chloroplast genome; DNA transfer; gene expression; Gramineae; mitochondrial genome;
D O I
10.1023/A:1005828728036
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We reported previously that the mitochondrial sequence that contains the chloroplast-derived trnH gene has been highly conserved in the region around one terminus of the junction between chloroplast-derived and mitochondrion-specific sequences in most of the gramineous plants analyzed [15]. The results of RT-PCR, northern hybridization, in vitro capping and ribonuclease protection experiments show that the chloroplast-derived trnH gene is transcribed from a putative promoter that is located in the mitochondrion-specific sequence. Gene expression in this region seems to be correlated with the conservation of the sequence at the junction between the chloroplast-derived fragment and the mitochondrion-specific sequence.
引用
收藏
页码:353 / 356
页数:4
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