DC photoelectric signals from bacteriorhodopsin adsorbed on lipid monolayers and thiol/lipid bilayers supported by mercury

被引：6

作者：

Dolfi, A ^{[1
]}

Buoninsegni, FT ^{[1
]}

Moncelli, MR ^{[1
]}

Guidelli, R ^{[1
]}

机构：

[1] Univ Florence, Dept Chem, I-50019 Sesto Fiorentino, Firenze, Italy

来源：

BIOELECTROCHEMISTRY | 2002年 / 56卷 / 1-2期

关键词：

bacteriorhodopsin; lipid monolayers; bilayers; mercury; proton transport;

D O I：

10.1016/S1567-5394(02)00014-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Purple membrane (PM) fragments were adsorbed on a dioleoylphosphatidylcholine (DOPC) monolayer and on a mixed alkanethiol/DOPC bilayer supported by mercury to investigate the kinetics of light-driven proton transport by bacteriorhodopsin (W). The light-on and light-off capacitive currents on an alkanethiol/DOPC bilayer at pH 6.4 were interpreted on the basis of a simple equivalent circuit. The pH dependence of the biphasic decay kinetics of the light-on currents was analyzed to estimate the pK(a) values for the transitions releasing protons to, and taking up protons from, the solution. The linear dependence of the stationary light-on current of bR on a DOPC monolayer self-assembled on mercury upon the applied potential was interpreted on the basis of an equivalent circuit. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：151 / 156

页数：6

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