Quantitative in situ assay of salicylic acid in tobacco leaves using a genetically modified biosensor strain of Acinetobacter sp ADP1

被引:87
作者
Huang, Wei E.
Huang, Linfeng
Preston, Gail M.
Naylor, Martin
Carr, John P.
Li, Yanhong
Singer, Andrew C.
Whiteley, Andrew S.
Wang, Hui
机构
[1] NERC, Ctr Ecol & Hydrol Oxford, Oxford OX1 3SR, England
[2] Univ Oxford, Dept Plant Sci, Oxford OX1 3RB, England
[3] Univ Cambridge, Dept Plant Sci, Cambridge CB2 3EA, England
[4] Capital Normal Univ, Coll Biol, Beijing 100037, Peoples R China
基金
英国自然环境研究理事会;
关键词
salicylic acid; biosensor; in situ assay; hypersensitive response; systemic acquired resistance; Acinetobacter sp ADP1;
D O I
10.1111/j.1365-313X.2006.02758.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Salicylic acid (SA) plays important roles in plants, most notably in the induction of systemic acquired resistance (SAR) against pathogens. A non-destructive in situ assay for SA would provide new insights into the functions of SA in SAR and other SA-regulated phenomena. We assessed a genetically engineered strain of Acinetobacter sp. ADP1, which proportionally produces bioluminescence in response to salicylates including SA and methylsalicylate, as a reporter for salicylate accumulation in the apoplast of plant leaves. SA was measured quantitatively in situ in NN genotype tobacco (Nicotiana tabacum L. cv Xanthi-nc) leaves inoculated with tobacco mosaic virus (TMV). The biosensor revealed accumulation of apoplastic SA before the visible appearance of hypersensitive response (HR) lesions. When the biosensor was infiltrated into TMV-inoculated leaves displaying HR lesions at 90 and 168 h post-inoculation, salicylate accumulation was detected predominantly in tissues surrounding the lesions and in veins adjacent to HR lesions. These images are consistent with previous data demonstrating that SA accumulation occurs prior to and following the onset of visible HR lesions. We also used the biosensor to observe apoplastic SA accumulation in tobacco leaves inoculated with virulent and HR-eliciting strains of the bacterial plant pathogen Pseudomonas syringae. The work demonstrates that the Acinetobacter sp. ADP1 biosensor is a useful new tool to non-destructively assay salicylates in situ and to map their spatial distribution in plant tissues.
引用
收藏
页码:1073 / 1083
页数:11
相关论文
共 48 条
[1]   Salicylic acid in the machinery of hypersensitive cell death and disease resistance [J].
Alvarez, ME .
PLANT MOLECULAR BIOLOGY, 2000, 44 (03) :429-442
[2]   Unique features revealed by the genome sequence of Acinetobacter sp ADP1, a versatile and naturally transformation competent bacterium [J].
Barbe, V ;
Vallenet, D ;
Fonknechten, N ;
Kreimeyer, A ;
Oztas, S ;
Labarre, L ;
Cruveiller, S ;
Robert, C ;
Duprat, S ;
Wincker, P ;
Ornston, LN ;
Weissenbach, J ;
Marlière, P ;
Cohen, GN ;
Médigue, C .
NUCLEIC ACIDS RESEARCH, 2004, 32 (19) :5766-5779
[3]   Biophoton imaging:: A nondestructive method for assaying R gene responses [J].
Bennett, M ;
Mehta, M ;
Grant, M .
MOLECULAR PLANT-MICROBE INTERACTIONS, 2005, 18 (02) :95-102
[4]   Intercellular salicylic acid accumulation is important for age-related resistance in Arabidopsis to Pseudomonas syringae [J].
Cameron, RK ;
Zaton, K .
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 2004, 65 (04) :197-209
[5]   Thermal and chlorophyll-fluorescence imaging distinguish plant-pathogen interactions at an early stage [J].
Chaerle, L ;
Hagenbeek, D ;
De Bruyne, E ;
Valcke, R ;
Van Der Straeten, D .
PLANT AND CELL PHYSIOLOGY, 2004, 45 (07) :887-896
[6]   Presymptomatic visualization of plant-virus interactions by thermography [J].
Chaerle, L ;
Van Caeneghem, W ;
Messens, E ;
Lambers, H ;
Van Montagu, M ;
Van Der Straeten, D .
NATURE BIOTECHNOLOGY, 1999, 17 (08) :813-816
[7]   BIOTECHNOLOGICAL APPLICATIONS OF BACTERIAL BIOLUMINESCENCE (LUX) GENES [J].
CHATTERJEE, J ;
MEIGHEN, EA .
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1995, 62 (04) :641-650
[8]   The formation, vacuolar localization, and tonoplast transport of salicylic acid glucose conjugates in tobacco cell suspension cultures [J].
Dean, JV ;
Mohammed, LA ;
Fitzpatrick, T .
PLANTA, 2005, 221 (02) :287-296
[9]   Systemic acquired resistance [J].
Durrant, WE ;
Dong, X .
ANNUAL REVIEW OF PHYTOPATHOLOGY, 2004, 42 :185-209
[10]   LOCALIZATION, CONJUGATION, AND FUNCTION OF SALICYLIC-ACID IN TOBACCO DURING THE HYPERSENSITIVE REACTION TO TOBACCO MOSAIC-VIRUS [J].
ENYEDI, AJ ;
YALPANI, N ;
SILVERMAN, P ;
RASKIN, I .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (06) :2480-2484