GTPγS-stimulated lysosomal lysis dependent on the assembly of adaptor protein on lysosome

被引：2

作者：

Arai, K ^{[1
]}

Yoshida, S ^{[1
]}

Naito, S ^{[1
]}

Ohkuma, S ^{[1
]}

机构：

[1] Kanazawa Univ, Fac Pharmaceut Sci, Lab Biochem & Mol Cell Biol, Dept Pharmaceut Biol, Kanazawa, Ishikawa 9200934, Japan

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2002年 / 25卷 / 09期

关键词：

adaptor protein; ADP-ribosylation factor; lysis; lysosome;

D O I：

10.1248/bpb.25.1125

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Cytosol treated with guanosine 5'-O-(3-thiotriphosphate) (GTPgammaS) destroys the dextran-loaded lysosomes (J Biochem., 123, 637 (1998)). The transfer of the ADP-ribosylation factor (ARF) from the cytosol to the lysosomal membrane is necessary for this lysis to occur. The role of ARF in the biogenesis of the Golgi complex is to generate high-affinity membrane-binding sites for the heterotetrameric adaptor protein complex in Golgi membranes. We have found that ARF also recruits the adaptor protein to lysosomes. The recruitment of protein coats for vesicles is necessary for the GTPgammaS-stimulated lysis of lysosomes. The GTPgammaS-induced lysis proceeded via a process similar to that for the assembly of coated proteins to coated vesicles, which serve to transport proteins between intracellular organelles.

引用

页码：1125 / 1128

页数：4

共 24 条

[1]

Arai K, 1998, J BIOCHEM-TOKYO, V123, P637

[2] Coupled inositide phosphorylation and phospholipase D activation initiates clathrin-coat assembly on lysosomes [J].