Expression and characterization of a trpl homolog from rat

被引：32

作者：

Preuss, KD ^{[1
]}

Noller, JK ^{[1
]}

Krause, E ^{[1
]}

Gobel, A ^{[1
]}

Schulz, I ^{[1
]}

机构：

[1] UNIV SAARLAND,INST PHYSIOL 2,D-66421 HOMBURG,GERMANY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 240卷 / 01期

关键词：

D O I：

10.1006/bbrc.1997.7528

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mammalian homologues of the Drosophila trp/trpl-gene-family code for ''Ca2+-store-operated'' channels. Here we describe the cloning and expression of a trp/trpl homologous gene from rat brain. The clone is named Rtrp3 because of its high homology to the recently described Htrp3 (Zhu et al (1996) Cell 85:661-671). Expression of Rtrp3 in the mammalian COS-1 cell line leads to 100% increase of capacitative Ca2+ entry as compared to the controls. This capacitative calcium entry can be completely blocked with La3+ (10 mu M). We conclude that Rtrp3, a new member of the growing family of trp/trpl homologues in mammalians, is involved in ''Ca2+-store-operated'' Ca2+ entry into the cell. (C) 1997 Academic Press.

引用

页码：167 / 172

页数：6

共 25 条

[1] GREEN FLUORESCENT PROTEIN [J].