The topology of phosphatidylglycerol populations is essential for sustaining photosynthetic electron flow activities in thylakoid membranes

被引:14
作者
Duchêne, S [1 ]
Smutny, J [1 ]
Siegenthaler, PA [1 ]
机构
[1] Univ Neuchatel, Lab Physiol Vegetale, CH-2007 Neuchatel, Switzerland
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2000年 / 1463卷 / 01期
关键词
lipid transmembrane distribution; thylakoid monolayer; outer/inner; phosphatidylglycerol; photosynthetic electron flow; Spinacia oleracea L; thylakoid inside-out vesicle;
D O I
10.1016/S0005-2736(99)00183-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The transmembrane distribution of phosphatidylglycerol (PG) was determined in rightside-out (RO) and inside-out vesicles (IO) obtained by fragmentation of spinach thylakoids in a Yeda press, followed by partition in an aqueous dextranpolyethyleneglycol two-phase system. Using the phospholipase A(2) from porcine pancreas to digest selectively PG molecules in the outer monolayer (exposed to the incubation medium) of the membrane, we found the molar outside/inside distribution to be 70/30 +/- 5 in RO and 40/60 +/- 3 in IO. The transmembrane distribution of PG in IO was the opposite of that in intact thylakoids (molar ratio 58/42 +/- 3). The phospholipid population which sustained most of the uncoupled photosystem II electron flow activity was localized in the inner monolayer (exposed to the thylakoid lumen) of both thylakoid and RO membranes. In contrast, the activity in IO membranes was highly dependent on the PG population located in the outer monolayer. This finding brings the first direct demonstration of the dependence of the photosynthetic electron flow activity on the integrity of the inner topological pool of PG in the thylakoid membrane. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:115 / 120
页数:6
相关论文
共 25 条
[1]   ISOLATION OF PHOTOSYSTEM-II ENRICHED MEMBRANE-VESICLES FROM SPINACH-CHLOROPLASTS BY PHASE PARTITION [J].
AKERLUND, HE ;
ANDERSSON, B ;
ALBERTSSON, PA .
BIOCHIMICA ET BIOPHYSICA ACTA, 1976, 449 (03) :525-535
[2]   QUANTITATIVE SEPARATION OF SPINACH THYLAKOIDS INTO PHOTOSYSTEM-II-ENRICHED INSIDE-OUT VESICLES AND PHOTOSYSTEM-I-ENRICHED RIGHT-SIDE-OUT VESICLES [J].
AKERLUND, HE ;
ANDERSSON, B .
BIOCHIMICA ET BIOPHYSICA ACTA, 1983, 725 (01) :34-40
[3]   The structure and function of the chloroplast photosynthetic membrane - A model for the domain organization [J].
Albertsson, PA .
PHOTOSYNTHESIS RESEARCH, 1995, 46 (1-2) :141-149
[4]   LIGHT-INDUCED REVERSIBLE PROTON EXTRUSION BY SPINACH-CHLOROPLAST PHOTOSYSTEM 2 VESICLES ISOLATED BY PHASE PARTITION [J].
ANDERSSON, B ;
AKERLUND, HE ;
ALBERTSSON, PA .
FEBS LETTERS, 1977, 77 (02) :141-145
[5]   INSIDE-OUT MEMBRANE-VESICLES ISOLATED FROM SPINACH THYLAKOIDS [J].
ANDERSSON, B ;
AKERLUND, HE .
BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 503 (03) :462-472
[6]   SEPARATION AND CHARACTERIZATION OF STROMA AND GRANA MEMBRANES - EVIDENCE FOR HETEROGENEITY IN ANTENNA SIZE OF BOTH PHOTOSYSTEM-I AND PHOTOSYSTEM-II [J].
ANDREASSON, E ;
SVENSSON, P ;
WEIBULL, C ;
ALBERTSSON, PA .
BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 936 (03) :339-350
[7]   A COMMENT ON SPECTROPHOTOMETRIC DETERMINATION OF CHLOROPHYLL [J].
BRUINSMA, J .
BIOCHIMICA ET BIOPHYSICA ACTA, 1961, 52 (03) :576-&
[8]   LOCALIZATION OF PHOSPHOLIPIDS IN THE MEMBRANE OF BACILLUS-MEGATERIUM [J].
DEMANT, EJF ;
OPDENKAMP, JAF ;
VANDEENEN, LLM .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1979, 95 (03) :613-619
[9]  
DUCHENE S, 1996, EXPERIENTIA, V52, pS8
[10]  
DUCHENE S, 1998, THESIS U NEUCHATEL N, P1