MOLECULAR RECOGNITION OF THE DISORDERED DIHYDROPYRIDINE RECEPTOR II-III LOOP BY A CONSERVED SPRY DOMAIN OF THE TYPE 1 RYANODINE RECEPTOR

被引：12

作者：

Tae, Han-Shen ^{[1
]}

Norris, Nicole C. ^{[1
]}

Cui, Yanfang ^{[1
]}

Karunasekara, Yamuna ^{[1
]}

Board, Philip G. ^{[1
]}

Dulhunty, Angela F. ^{[1
]}

Casarotto, Marco G. ^{[1
]}

机构：

[1] Australian Natl Univ, John Curtin Sch Med Res, Div Mol Biosci, Canberra, ACT 2601, Australia

来源：

CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY | 2009年 / 36卷 / 03期

关键词：

dihydropyridine receptor; II-III loop; nuclear magnetic resonance; protein-protein interaction; ryanodine receptor; SKELETAL-MUSCLE; CA2+ RELEASE; C-REGION; CONTRACTION; ACTIVATION; IDENTIFICATION; IMPERATOXIN; PEPTIDES; CHANNELS; SITE;

D O I：

10.1111/j.1440-1681.2008.05130.x

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

1. The dihydropyridine receptor (DHPR) II-III loop is an intrinsically unstructured region made up of alpha-helical and beta-turn secondary structure elements with the N and C termini in close spatial proximity. 2. The DHPR II-III loop interacts in vitro with a ryanodine receptor (RyR) 1 SPRY domain through alpha-helical segments located in the A and B regions. Mutations within the A and B regions in the DHPR II-III loop alter the binding affinity to the SPRY2 domain. 3. The A and C peptides derived from DHPR II-III loop show negative cooperativity in binding to the SPRY2 domain. 4. The SPRY2 domain of the RyR1 (1085-1208) forms a beta-sheet sandwich structure flanked by variable loop regions. An acidic loop region of SPRY2 (1107-1121) forms part of a negatively charged cleft that is implicated in the binding of the DHPR II-III loop. 5. The mutant E1108A located in the negatively charged loop of SPRY2 reduces the binding affinity to the DHPR II-III loop.

引用

页码：346 / 349

页数：4

共 29 条

[1] The mitochondrial ryanodine receptor in rat heart: A pharmaco-kinetic profile [J].

Altschafl, Beth A. ;

Beutner, Gisela ;

Sharma, Virendra K. ;

Sheu, Shey-Shing ;

Valdivia, Hctor H. .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2007, 1768 (07) :1784-1795

[2] Structural and functional characterization of interactions between the dihydropyridine receptor II-III loop and the ryanodine receptor [J].

Casarotto, Marco G. ;

Cui, Yanfang ;

Karunasekara, Yamuna ;

Harvey, Peta J. ;

Norris, Nicole ;

Board, Philip G. ;

Dulhunty, Angela F. .

CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY, 2006, 33 (11) :1114-1117

[3] A structural requirement for activation of skeletal ryanodine receptors by peptides of the dihydropyridine receptor II-III loop [J].

Casarotto, MG ;

Gibson, F ;

Pace, SM ;

Curtis, SM ;

Mulcair, M ;

Dulhunty, AF .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (16) :11631-11637

[4] Maurocalcine and peptide A stabilize distinct subconductance states of ryanodine receptor type 1, revealing a proportional gating mechanism [J].

Chen, L ;

Estève, E ;

Sabatier, JM ;

Ronjat, M ;

De Waard, M ;

Allen, PD ;

Pessah, IN .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (18) :16095-16106

[5] A dihydropyridine receptor α1s loop region critical for skeletal muscle contraction is intrinsically unstructured and binds to a SPRY domain of the type 1 ryanodine receptor [J].

Cui, Yanfang ;

Tae, Han-Shen ;

Norris, Nicole C. ;

Karunasekara, Yamuna ;

Pouliquin, Pierre ;

Board, Philip G. ;

Dulhunty, Angela F. ;

Casarotto, Marco G. .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2009, 41 (03) :677-686

[6] Role of some unconserved residues in the "C" region of the skeletal DHPR II-III loop [J].

Dulhunty, AF ;

Karunasekara, Y ;

Curtis, SM ;

Harvey, PJ ;

Board, PG ;

Casarotto, MG .

FRONTIERS IN BIOSCIENCE, 2005, 10 :1368-1381

[7] The recombinant dihydropyridine receptor II-III loop and partly structured 'C' region peptides modify cardiac ryanodine receptor activity [J].

Dulhunty, AF ;

Karunasekara, Y ;

Curtis, SM ;

Harvey, PJ ;

Board, PG ;

Casarotto, MG .

BIOCHEMICAL JOURNAL, 2005, 385 :803-813

[8] Interactions between dihydropyridine receptors and ryanodine receptors in striated muscle [J].

Dulhunty, AF ;

Haarmann, CS ;

Green, D ;

Laver, DR ;

Board, PG ;

Casarotto, MG .

PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, 2002, 79 (1-3) :45-75

[9] Activation and inhibition of skeletal RyR channels by a part of the skeletal DHPR II-III loop:: Effects of DHPR Ser687 and FKBP12 [J].

Dulhunty, AF ;

Laver, DR ;

Gallant, EM ;

Casarotto, MG ;

Pace, SM ;

Curtis, S .

BIOPHYSICAL JOURNAL, 1999, 77 (01) :189-203

[10] Profile hidden Markov models [J].

Eddy, SR .

BIOINFORMATICS, 1998, 14 (09) :755-763

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