Analysis of ammonia-oxidizing bacteria populations in acid forest soil during conditions of moisture limitation

被引:52
作者
Hastings, RC
Butler, C
Singleton, I
Saunders, JR
McCarthy, AJ [1 ]
机构
[1] Univ Liverpool, Sch Biol Sci, Liverpool L69 7ZB, Merseyside, England
[2] Univ Coll Dublin, Dept Ind Microbiol, Dublin, Ireland
[3] Univ Adelaide, Dept Soil & Water, Adelaide, SA, Australia
关键词
D O I
10.1046/j.1472-765x.2000.00630.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Ammonia-oxidizer numbers decreased under conditions of moisture limitation in litter, fermentation and humus layers of forest soil in the field, but the extent of regrowth after rehydration varied between layers. Nitrosospira 16S rRNA genes were amplified from all layers, regardless of moisture content or soil pH which varied between 4.1 and 5.2. Nitrosomonas spp. were detected less often, but appeared to exhibit mol-e rapid recovery than the Nitrosospira spp, when drought conditions were relieved by rainfall.
引用
收藏
页码:14 / 18
页数:5
相关论文
共 25 条
[1]  
BOCK E, 1986, NITRIFICATION, P27
[2]   ISOLATION AND DIRECT COMPLETE NUCLEOTIDE DETERMINATION OF ENTIRE GENES - CHARACTERIZATION OF A GENE CODING FOR 16S-RIBOSOMAL RNA [J].
EDWARDS, U ;
ROGALL, T ;
BLOCKER, H ;
EMDE, M ;
BOTTGER, EC .
NUCLEIC ACIDS RESEARCH, 1989, 17 (19) :7843-7853
[3]   Direct molecular biological analysis of ammonia oxidising bacteria populations in cultivated soil plots treated with swine manure [J].
Hastings, RC ;
Ceccherini, MT ;
Miclaus, N ;
Saunders, JR ;
Bazzicalupo, M ;
McCarthy, AJ .
FEMS MICROBIOLOGY ECOLOGY, 1997, 23 (01) :45-54
[4]  
Hastings RC, 1998, APPL ENVIRON MICROB, V64, P3674
[5]  
HAYATSU M, 1991, PROCEEDINGS OF THE INTERNATIONAL SYMPOSIUM ON TEA SCIENCE, P757
[6]  
HAYES RJ, 1989, J SOIL SCI, V40, P341
[7]  
Haynes R. J., 1986, Mineral nitrogen in the plant-soil system., P127
[8]   AMPLIFICATION OF 16S RIBOSOMAL-RNA GENES OF AUTOTROPHIC AMMONIA-OXIDIZING BACTERIA DEMONSTRATES THE UBIQUITY OF NITROSOSPIRAS IN THE ENVIRONMENT [J].
HIORNS, WD ;
HASTINGS, RC ;
HEAD, IM ;
MCCARTHY, AJ ;
SAUNDERS, JR ;
PICKUP, RW ;
HALL, GH .
MICROBIOLOGY-SGM, 1995, 141 :2793-2800
[9]  
Kowalchuk GA, 1999, APPL ENVIRON MICROB, V65, P396
[10]   Analysis of ammonia-oxidizing bacteria of the beta subdivision of the class Proteobacteria in coastal sand dunes by denaturing gradient gel electrophoresis and sequencing of PCR-amplified 16S ribosomal DNA fragments [J].
Kowalchuk, GA ;
Stephen, JR ;
DeBoer, W ;
Prosser, JI ;
Embley, TM ;
Woldendorp, JW .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (04) :1489-1497