Klotho expression in cervical cancer: differential expression in adenocarcinoma and squamous cell carcinoma

被引:39
作者
Aviel-Ronen, Sarit [1 ,2 ]
Rubinek, Tami [3 ]
Zadok, Oranit [1 ]
Vituri, Aya [4 ]
Avivi, Camila [1 ]
Wolf, Ido [3 ,5 ]
Barshack, Iris [1 ,5 ]
机构
[1] Sheba Med Ctr, Dept Pathol, IL-52621 Tel Hashomer, Israel
[2] Sheba Med Ctr, Talpiot Med Leadership Program, IL-52621 Tel Hashomer, Israel
[3] Tel Aviv Univ, Tel Aviv Sourasky Med Ctr, Inst Oncol, IL-69978 Tel Aviv, Israel
[4] Tel Aviv Univ, Dept Stat & Operat Res, IL-69978 Tel Aviv, Israel
[5] Tel Aviv Univ, Sackler Fac Med, IL-69978 Tel Aviv, Israel
关键词
TUMOR-SUPPRESSOR; LUNG-CANCER; INVASION; GROWTH; PROGRESSION; APOPTOSIS; CLEAVAGE; MUTATION; PATHWAY; CATENIN;
D O I
10.1136/jclinpath-2015-202929
中图分类号
R36 [病理学];
学科分类号
100103 [病原生物学];
摘要
Aims Klotho is a trans-membrane protein that serves as a tumour suppressor in a wide array of malignancies. Recent data suggest it as an epigenetically silenced tumour suppressor in cervical cancer. Yet, the expression pattern of klotho in cervical cancer has not been determined. We aimed to study the expression of klotho in squamous cell carcinomas (SQCC) and adenocarcinoma ( ADC) of the cervix. Methods Klotho expression was analysed by immunohistochemistry in 44 SQCC samples, 38 ADC samples and the adjacent normal tissue. For each sample, percentage of positive stained cells, staining intensity and a combined staining score were recorded. Staining was validated by measuring klotho mRNA levels, using quantitative RT-PCR, in 18 of the samples. Results Klotho expression was high in all endocervical and exocervical normal tissues adjacent to tumour. No expression of klotho was noted in 7 out of 38 (18.4%) ADC samples and in 2 out of 44 (4.5%) SQCC samples. Staining intensity, number of positively stained cells and combined intensity score were all lower in tumours compared with normal adjacent tissues in ADC and SQCC. Klotho mRNA levels highly correlated with immunohistochemical (IHC) staining (p=0.008). Conclusions We found reduced klotho expression in cervical carcinoma, especially in ADC, compared with normal adjacent tissue. Our results support the role of klotho as a potential tumour suppressor in cervical cancer. Further studies are required in order to establish the therapeutic role of klotho in cervical carcinoma and identify patients who may benefit from it.
引用
收藏
页码:53 / 57
页数:5
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