Deformability study of breast cancer cells using microfluidics

被引:277
作者
Hou, H. W. [2 ]
Li, Q. S. [3 ]
Lee, G. Y. H. [4 ]
Kumar, A. P. [5 ]
Ong, C. N. [1 ,6 ]
Lim, C. T. [1 ,2 ,3 ,4 ]
机构
[1] Natl Univ Singapore, Inst Life Sci, Singapore 117548, Singapore
[2] Natl Univ Singapore, Grad Sch Integrat Sci & Engn, Singapore 117548, Singapore
[3] Natl Univ Singapore, Dept Mech Engn, Singapore 117548, Singapore
[4] Singapore MIT Alliance, Singapore, Singapore
[5] Natl Univ Singapore, Natl Univ Med Inst, Singapore 117548, Singapore
[6] Natl Univ Singapore, Dept Community Occupat & Family Med, Singapore 117548, Singapore
关键词
Microfluidics; Breast tumor cells; Metastasis; Biorheology; Cell deformability; Cell mechanics; BIOMECHANICS; DEFORMATION; NUCLEUS;
D O I
10.1007/s10544-008-9262-8
中图分类号
R318 [生物医学工程];
学科分类号
100103 [病原生物学];
摘要
Cell deformability is an important biomarker which can be used to distinguish between healthy and diseased cells. In this study, microfluidics is used to probe the biorheological behaviour of breast cancer cells in an attempt to develop a method to distinguish between non-malignant and malignant cells. A microfabricated fluidic channel design consisting of a straight channel and two reservoirs was used to study the biorheological behaviour of benign breast epithelial cells (MCF-10A) and non-metastatic tumor breast cells (MCF-7). Quantitative parameters such as entry time (time taken for the cell to squeeze into the microchannel) and transit velocity (speed of the cell flowing through the microchannel) were defined and measured from these studies. Our results demonstrated that a simple microfluidic device can be used to distinguish the difference in stiffness between benign and cancerous breast cells. This work lays the foundation for the development of potential microfluidic devices which can subsequently be used in the detection of cancer cells.
引用
收藏
页码:557 / 564
页数:8
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