Artificial enzyme mimics have attracted considerable interest due to easy denaturation and leakage of enzymes during their storage and immobilization procedure. Herein we describe the design of a novel mimic peroxidase, a nanostructure of sheet-like FeS prepared by a simple micelle-assisted synthetic method. Such a nanostructure has a large specific surface area and high peroxidase-like activity, and was thus further used as a mimic enzyme for the development of biocatalysts and amperometric biosensors. The sheet-like FeS nanostructure showed typical Michaelis-Menten kinetics and good affinity to both H2O2 and 3,3',5,5'-tetramethyl benzidine. At pH 7.0 the constructed amperometric sensor showed a linear range for the detection of H2O2 from 0.5 to 150 mu M with a correlation coefficient of 0.9998 without any electron transfer mediator. ne H2O2 sensor based on the sheet-like FeS showed more sensitive response than those based on spherical FeS nanostructure, and resulted in a better stability than horseradish peroxidase when they were exposed to solutions with different pH values and temperatures. These excellent properties made the sheet-like nanostructured FeS powerful tools for a wide range of potential applications as ail "artificial peroxidase" as biosensors and biotechnology.
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Northwestern Univ, Dept Chem, Evanston, IL 60208 USANorthwestern Univ, Dept Chem, Evanston, IL 60208 USA
Banholzer, Matthew J.
Millstone, Jill E.
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Northwestern Univ, Dept Chem, Evanston, IL 60208 USANorthwestern Univ, Dept Chem, Evanston, IL 60208 USA
Millstone, Jill E.
Qin, Lidong
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Northwestern Univ, Dept Chem, Evanston, IL 60208 USANorthwestern Univ, Dept Chem, Evanston, IL 60208 USA
Qin, Lidong
Mirkin, Chad A.
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机构:
Northwestern Univ, Dept Chem, Evanston, IL 60208 USA
Northwestern Univ, Int Inst Nanotechnol, Evanston, IL 60208 USANorthwestern Univ, Dept Chem, Evanston, IL 60208 USA
机构:
Northwestern Univ, Dept Chem, Evanston, IL 60208 USANorthwestern Univ, Dept Chem, Evanston, IL 60208 USA
Banholzer, Matthew J.
Millstone, Jill E.
论文数: 0引用数: 0
h-index: 0
机构:
Northwestern Univ, Dept Chem, Evanston, IL 60208 USANorthwestern Univ, Dept Chem, Evanston, IL 60208 USA
Millstone, Jill E.
Qin, Lidong
论文数: 0引用数: 0
h-index: 0
机构:
Northwestern Univ, Dept Chem, Evanston, IL 60208 USANorthwestern Univ, Dept Chem, Evanston, IL 60208 USA
Qin, Lidong
Mirkin, Chad A.
论文数: 0引用数: 0
h-index: 0
机构:
Northwestern Univ, Dept Chem, Evanston, IL 60208 USA
Northwestern Univ, Int Inst Nanotechnol, Evanston, IL 60208 USANorthwestern Univ, Dept Chem, Evanston, IL 60208 USA