Nanostructured FeS as a Mimic Peroxidase for Biocatalysis and Biosensing

被引:292
作者
Dai, Zhihui [1 ]
Liu, Shaohua [1 ]
Bao, Jianchun [1 ]
Jui, Huangxian [2 ]
机构
[1] Nanjing Normal Univ, Coll Chem & Environm Sci, Jiangsu Key Lab Biofunct Mat, Nanjing, Peoples R China
[2] Nanjing Univ, Dept Chem, MOE Key Lab Analyt Chem Life Sci, Nanjing 210093, Peoples R China
基金
中国国家自然科学基金;
关键词
amperometric biosensors; biosensors; enzyme catalysis; FeS nanostructure; peroxide mimic; DIRECT ELECTRON-TRANSFER; PRUSSIAN-BLUE; HYDROGEN-PEROXIDE; HORSERADISH-PEROXIDASE; CARBON NANOTUBES; NANOPARTICLES; MATRIX; H2O2; ELECTROCHEMISTRY; IMMOBILIZATION;
D O I
10.1002/chem.200802158
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Artificial enzyme mimics have attracted considerable interest due to easy denaturation and leakage of enzymes during their storage and immobilization procedure. Herein we describe the design of a novel mimic peroxidase, a nanostructure of sheet-like FeS prepared by a simple micelle-assisted synthetic method. Such a nanostructure has a large specific surface area and high peroxidase-like activity, and was thus further used as a mimic enzyme for the development of biocatalysts and amperometric biosensors. The sheet-like FeS nanostructure showed typical Michaelis-Menten kinetics and good affinity to both H2O2 and 3,3',5,5'-tetramethyl benzidine. At pH 7.0 the constructed amperometric sensor showed a linear range for the detection of H2O2 from 0.5 to 150 mu M with a correlation coefficient of 0.9998 without any electron transfer mediator. ne H2O2 sensor based on the sheet-like FeS showed more sensitive response than those based on spherical FeS nanostructure, and resulted in a better stability than horseradish peroxidase when they were exposed to solutions with different pH values and temperatures. These excellent properties made the sheet-like nanostructured FeS powerful tools for a wide range of potential applications as ail "artificial peroxidase" as biosensors and biotechnology.
引用
收藏
页码:4321 / 4326
页数:6
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