Inactivated meningococci and pertussis bacteria are immunogenic and act as mucosal adjuvants for a nasal inactivated influenza virus vaccine

被引:32
作者
Berstad, AKH
Andersen, SR
Dalseg, R
Dromtorp, S
Holst, J
Namork, E
Wedege, E
Haneberg, B
机构
[1] Natl Publ Hlth Inst, Dept Vaccinol, N-0403 Oslo, Norway
[2] Natl Publ Hlth Inst, Dept Environm Med, N-0403 Oslo, Norway
[3] Univ Oslo, Inst Pharm, Dept Microbiol, Oslo, Norway
关键词
adjuvant; intranasal; influenza vaccine; meningococci; pertussis;
D O I
10.1016/S0264-410X(99)00442-9
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Whole killed meningococci (Nm) and pertussis bacteria (Bp) were tested for mucosal immunogenicity and as mucosal adjuvants for an inactivated influenza virus vaccine given intranasally to unanaesthetized mice. Virus was given alone, or simply mixed with one of the bacterial preparations, in four doses at weekly intervals. The virus alone induced low but significant increases of influenza-specific IgG antibodies iu serum measured by ELISA, whereas IgA responses in serum and saliva were insignificant compared to non-immunized controls. With Bp or Nm admired, serum Ige and IgA and salivary IgA responses to the influenza virus were substantially augmented (P < 0.005). However, this adjuvant effect of the bacterial preparations was not significant for responses in the intestine as measured by antibodies in faeces. Antibody responses to Bp itself but not to Nm, were inhibited by the admixture of the virus vaccine. Moreover, the pertussis preparation induced salivary antibodies which cross-reacted with Nm. Whole-cell bacteria with inherent strong mucosal immunogenicity may also possess mucosal adjuvanticity for admired particulate antigens which are weakly immunogenic by the nasal route. (C) 2000 Elsevier Science Ltd, All rights reserved.
引用
收藏
页码:1910 / 1919
页数:10
相关论文
共 46 条
[1]  
AABERGE IS, 1995, J CELL BIOCH A S, V19, P242
[2]  
BENAHMEIDA ETS, 1993, VACCINE, V11, P1302
[3]   Anticarrier immunity suppresses the antibody response to polysaccharide antigens after intranasal immunization with the polysaccharide-protein conjugate [J].
Bergquist, C ;
Lagergard, T ;
Holmgren, J .
INFECTION AND IMMUNITY, 1997, 65 (05) :1579-1583
[4]  
Berstad AKH, 1997, VACCINE, V15, P1473
[5]  
BERSTAD AKH, 1999, J MED MICROBIOL, P48
[6]   HUMORAL IMMUNE-RESPONSE PATTERNS OF HUMAN MUCOSAE - INDUCTION AND RELATION TO BACTERIAL RESPIRATORY-TRACT INFECTIONS [J].
BRANDTZAEG, P .
JOURNAL OF INFECTIOUS DISEASES, 1992, 165 :S167-S176
[7]  
BRENNAN MJ, 1991, J BIOL CHEM, V266, P18827
[8]   PARENTERAL INFLUENZA VACCINATION INDUCES A RAPID SYSTEMIC AND LOCAL IMMUNE-RESPONSE [J].
BROKSTAD, KA ;
COX, RJ ;
OLOFSSON, J ;
JONSSON, R ;
LARS, R .
JOURNAL OF INFECTIOUS DISEASES, 1995, 171 (01) :198-203
[9]   DEVELOPMENT AND PERSISTENCE OF LOCAL AND SYSTEMIC ANTIBODY-RESPONSES IN ADULTS GIVEN LIVE ATTENUATED OR INACTIVATED INFLUENZA-A VIRUS-VACCINE [J].
CLEMENTS, ML ;
MURPHY, BR .
JOURNAL OF CLINICAL MICROBIOLOGY, 1986, 23 (01) :66-72
[10]   Effective immunization with live attenuated influenza A virus can be achieved in early infancy [J].
Clements, ML ;
Makhene, MK ;
Karron, RA ;
Murphy, BR ;
Steinhoff, MC ;
Subbarao, K ;
Wilson, MH ;
Wright, PF .
JOURNAL OF INFECTIOUS DISEASES, 1996, 173 (01) :44-51