Development of molecular and biochemical tools to investigate fruit quality traits in strawberry elite genotypes

被引：29

作者：

Carbone, Fabrizio

Mourgues, Fabienne

Biasioli, Franco

Gasperi, Flavia

Maerk, Tilmann D.

Rosati, Carlo

Perrotta, Gaetano

机构：

[1] ENEA, Trisaia Res Ctr, I-75026 Rotondella, MT, Italy

[2] Ist Agr S Michele all Adige, I-38010 S Michele, TN, Italy

[3] Univ Innsbruck, Inst Ionenphys, A-6020 Innsbruck, Austria

来源：

MOLECULAR BREEDING | 2006年 / 18卷 / 02期

基金：

以色列科学基金会;

关键词：

comparative gene expression profiling; enzyme assays; fruit quality; functional genomics; proton transfer reaction-mass spectrometry;

D O I：

10.1007/s11032-006-9017-2

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Molecular and biochemical studies were undertaken to elucidate gene/product relationships which influence key fruit quality traits in cultivated strawberry (Fragaria x ananassa Duch.). Comparative transcription profiling experiments in selected genotypes pointed out a number of differentially-expressed genes, possibly related to important fruit quality traits as aroma and fruit firmness. Some of the altered cDNAs encode putative cinnamyl alcohol dehydrogenase, cinnamoyl CoA reductase, cellulase and expansin genes, involved in the early steps of lignin biosynthesis and in cell wall structure, respectively. Parallel biochemical analyses studied the spectra of volatile compounds by Proton Transfer Reaction-Mass Spectrometry (PTR-MS) and alcohol acyl transferase (AAT) specific activity in red fruits. A correlation between the expression of an aat gene, total AAT activity and the presence of related esters in fruit headspace was found.

引用

收藏

页码：127 / 142

页数：16

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