Bacterial expression and purification of biologically active mouse c-Fos proteins by selective codon optimization

被引：17

作者：

Deng, TL ^{[1
]}

机构：

[1] UNIV FLORIDA, COLL MED, CTR MAMMALIAN GENET, GAINESVILLE, FL 32610 USA

来源：

FEBS LETTERS | 1997年 / 409卷 / 02期

关键词：

high-level bacterial expression; mouse c-Fos; selective codon optimization;

D O I：

10.1016/S0014-5793(97)00522-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple strategy using selective codon optimization was devised to express mouse c-Fos protein in high levels in E. coli. Ten arginine codons located in the basic region were optimized to achieve high levels of protein expression. The c-Fos protein was purified to near homogeneity and was demonstrated to be biologically active by assaying its several biological activities. (C) 1997 Federation of European Biochemical Societies.

引用

页码：269 / 272

页数：4

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