General Peroxidase Activity of G-Quadruplex-Hemin Complexes and Its Application in Ligand Screening

被引：252

作者：

Cheng, Xiaohong ^{[1
,2
]}

Liu, Xiangjun ^{[1
]}

Bing, Tao ^{[1
,2
]}

Cao, Zehui ^{[1
]}

Shangguan, Dihua ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

来源：

BIOCHEMISTRY | 2009年 / 48卷 / 33期

基金：

美国国家科学基金会;

关键词：

PROXIMAL PROMOTER REGION; HUMAN GENOME; DNA APTAMER; COLORIMETRIC DETECTION; MASS-SPECTROMETRY; RECOGNITION; DNAZYME; SEQUENCE; PORPHYRINS; STABILITY;

D O I：

10.1021/bi9006786

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA sequences with repetitive G-rich structural motifs, which form special structures called G-quadruplexes, widely exist in the human genome. Here we report the general peroxidase activity of G-quadruplex-hemin complexes and discuss the connection between peroxidase activity and G-quadruplex structures. The high peroxidase activity of hemin complexed with intramolecular parallel G-quadruplex-forming sequences in gene promoters (such as c-Myc, VEGF, c-Kit21, HIF-1 alpha, and RET) may imply a potential mechanism of hemin-mediated cellular injury. This peroxidase activity has also been demonstrated to be applicable for screening G-quadruplex ligands (potential anticancer reagents) using colorimetric and Visual detection strategies.

引用

页码：7817 / 7823

页数：7

共 51 条

[1]

AFT RL, 1983, J BIOL CHEM, V258, P2069

[2] Human telomeric sequence forms a hybrid-type intramolecular G-quadruplex structure with mixed parallel/antiparallel strands in potassium solution [J].

Ambrus, Attila ;

Chen, Ding ;

Dai, Jixun ;

Bialis, Tiffanie ;

Jones, Roger A. ;

Yang, Danzhou .

NUCLEIC ACIDS RESEARCH, 2006, 34 (09) :2723-2735

[3]

Armond R. D., 2005, BIOCHEMISTRY-US, V44, P16341

[4] The chemistry and biochemistry of heme c: functional bases for covalent attachment [J].

Bowman, Sarah E. J. ;

Bren, Kara L. .

NATURAL PRODUCT REPORTS, 2008, 25 (06) :1118-1130

[5] A sequence-independent study of the influence of short loop lengths on the stability and topology of intramolecular DNA G-quadruplexes [J].

Bugaut, Anthony ;

Balasubramanian, Shankar .

BIOCHEMISTRY, 2008, 47 (02) :689-697

[6] Quadruplex DNA: sequence, topology and structure [J].

Burge, Sarah ;

Parkinson, Gary N. ;

Hazel, Pascale ;

Todd, Alan K. ;

Neidle, Stephen .

NUCLEIC ACIDS RESEARCH, 2006, 34 (19) :5402-5415

[7] NMR solution structure of the major G-quadruplex structure formed in the human BCL2 promoter region [J].

Dai, Jixun ;

Chen, Ding ;

Jones, Roger A. ;

Hurley, Laurence H. ;

Yang, Danzhou .

NUCLEIC ACIDS RESEARCH, 2006, 34 (18) :5133-5144

[8] G-quartets 40 years later:: From 5′-GMP to molecular biology and supramolecular chemistry [J].

Davis, JT .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (06) :668-698

[9] Highly effective colorimetric and visual detection of nucleic acids using an asymmetrically split peroxidase DNAzyme [J].

Deng, Minggang ;

Zhang, Dan ;

Zhou, Yangyang ;

Zhou, Xiang .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (39) :13095-13102

[10] Gene function correlates with potential for G4 DNA formation in the human genome [J].

Eddy, Johanna ;

Maizels, Nancy .

NUCLEIC ACIDS RESEARCH, 2006, 34 (14) :3887-3896

← 1 2 3 4 5 6 →