Estrogen receptor mRNA in mineralized tissues of rainbow trout: Calcium mobilization by estrogen

被引：24

作者：

Armour, KJ

Lehane, DB

Pakdel, F

Valotaire, Y

Graham, R

Russell, G

Henderson, IW

机构：

[1] UNIV SHEFFIELD,DEPT ANIM & PLANT SCI,SHEFFIELD S10 2TN,S YORKSHIRE,ENGLAND

[2] UNIV SHEFFIELD,SCH MED,DEPT HUMAN METAB & CLIN BIOCHEM,SHEFFIELD S10 2RX,S YORKSHIRE,ENGLAND

[3] UNIV RENNES 1,UPRESA CNRS 256,MOL BIOL LAB,F-35042 RENNES,FRANCE

来源：

FEBS LETTERS | 1997年 / 411卷 / 01期

基金：

英国惠康基金;

关键词：

17; beta-estradiol; estrogen receptor; teleost; bone; scale;

D O I：

10.1016/S0014-5793(97)00680-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

RT-PCR was undertaken on total RNA extracts from bone and scales of the rainbow trout, Oncorhynchus mykiss., The rainbow trout estrogen receptor (ER)-specific primers used amplified a single product of expected size from each tissue which, using Southern blotting, strongly hybridized with a P-32-labelled rtER probe under stringent conditions, These data provide the first in vivo evidence of ER mRNA in bone and scale tissues of rainbow trout and suggest that the effects of estrogen observed in this study (increased bone mineral and decreased scale mineral contents, respectively) may be mediated directly through ER. (C) 1997 Federation of European Biochemical Societies.

引用

页码：145 / 148

页数：4

共 37 条

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[2]

DETECTION OF ESTROGEN-RECEPTOR MESSENGER-RNA IN TROUT PINEAL AND RETINA - ESTRADIOL-17-BETA MODULATES MELATONIN PRODUCTION BY CULTURED PINEAL PHOTORECEPTOR CELLS [J].