A fluoroimmunoassay based on immunoliposomes containing genetically engineered lipid-tagged antibody

被引：13

作者：

Kobatake, E

Sasakura, H

Haruyama, T

Laukkanen, ML

Keinanen, K

Aizawa, M

机构：

[1] TOKYO INST TECHNOL, FAC BIOSCI & BIOTECHNOL, DEPT BIOENGN, MIDORI KU, YOKOHAMA, KANAGAWA 226, JAPAN

[2] VTT BIOTECHNOL & FOOD RES, FIN-02044 ESPOO, FINLAND

来源：

ANALYTICAL CHEMISTRY | 1997年 / 69卷 / 07期

关键词：

D O I：

10.1021/ac961162+

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Immunoliposomes were prepared by using biosynthetically lipid-tagged anti-2-phenyloxazolone single-chain antibody. Carboxyfluorescein as a fluorescent marker was encapsulated in the immunoliposomes. Some conditions for fluoroimmunoassay using the immunoliposomes were optimized by binding assays with hapten-coated microtiter wells. A competitive fluoroimmunoassay for the caproic acid conjugate of 2-phenyloxazolone as a model antigen was performed with the immunoliposomes. In the optimized assay conditions, antigen could be determined in the concentration range from 10(-7) to 10(-9) M.

引用

页码：1295 / 1298

页数：4

共 27 条

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