Cathodic electrochemiluminescence of luminol enhanced by antibody-antigen reaction

被引：12

作者：

Yoshimi, Y ^{[1
]}

Haramoto, H ^{[1
]}

Miyasaka, T ^{[1
]}

Sakai, K ^{[1
]}

机构：

[1] WASEDA UNIV,DEPT CHEM ENGN,TOKYO 169,JAPAN

来源：

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN | 1996年 / 29卷 / 05期

关键词：

biochemical engineering; electrochemiluminescence; luminol; antigen; antibody;

D O I：

10.1252/jcej.29.851

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

The purpose of the present work is to characterize the cathodic electrochemiluminescence of luminol with a view to its application in continuous immunoassay. The authors designed a method for continuous antibody determination from the change in electrochemiluminescent intensity of luminol resulted from antigen-antibody reactions. The cathodic electrochemiluminescences of luminol-labeled antigens in hydrogen peroxide solution were enhanced by the presence of the respective antibodies. Cathodic electrochemiluminescence Of a luminol-labeled antibody was also enhanced by the presence of antigen. The enhancement of electrochemiluminescence may be useful in homogeneous immunoassay of antibodies or antigens. Analysis of a batchwise reaction suggested that the presence of antibody increases the quantum yield of electrochemiluminescence of luminol-labeled antigen. And analysis of the electrolytic current of luminol and hydrogen peroxide at indium-tin oxide (ITO) cathode revealed that hydrogen peroxide was electrochemically active at the cathode, while luminol was inert. These results suggest that cathodic electrochemiluminescence of luminol or luminol-labeled proteins is triggered by hydroxide radical produced from hydrogen peroxide at the ITO cathode.

引用

页码：851 / 857

页数：7

共 18 条

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Bard A.J, 1980, Electrochemical methods: Fundamentals and applications

[2]

EPSTEIN B, 1965, PHOTOCHEM PHOTOBIOL, V4, P1157

[3] CATHODIC ELECTROLUMINESCENCE AT THE TERBIUM(III)-DOPED OXIDE-COVERED TANTALUM ELECTRODE AND ITS FEASIBILITY FOR THE DETERMINATION OF HYDROGEN-PEROXIDE [J].