Collagen scaffold microenvironments modulate cell lineage commitment for differentiation of bone marrow cells into regulatory dendritic cells

被引：13

作者：

Fang, Yongxiang ^{[1
]}

Wang, Bin ^{[2
]}

Zhao, Yannan ^{[2
]}

Xiao, Zhifeng ^{[2
]}

Li, Jing ^{[2
]}

Cui, Yi ^{[2
,3
]}

Han, Sufang ^{[2
]}

Wei, Jianshu ^{[2
]}

Chen, Bing ^{[2
]}

Han, Jin ^{[2
]}

Meng, Qingyuan ^{[2
]}

Hou, Xianglin ^{[2
]}

Luo, Jianxun ^{[1
]}

Dai, Jianwu ^{[2
]}

Jing, Zhizhong ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Key Lab Vet Publ Hlth,Agr Minist, Lanzhou 730046, Peoples R China

[2] Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, Beijing 100190, Peoples R China

[3] Natl Res Inst Family Planning, Reprod & Genet Ctr, Beijing 100191, Peoples R China

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

基金：

中国国家自然科学基金;

关键词：

TGF-BETA; STEM-CELLS; T-CELLS; GROWTH; INDUCE; BIOMATERIALS; STIMULATION; MAINTENANCE; GENERATION; EXPRESSION;

D O I：

10.1038/srep42049

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

The microenvironment plays a pivotal role for cell survival and functional regulation, and directs the cell fate determination. The biological functions of DCs have been extensively investigated to date. However, the influences of the microenvironment on the differentiation of bone marrow cells (BMCs) into dendritic cells (DCs) are not well defined. Here, we established a 3D collagen scaffold microenvironment to investigate whether such 3D collagen scaffolds could provide a favourable niche for BMCs to differentiate into specialised DCs. We found that BMCs embedded in the 3D collagen scaffold differentiated into a distinct subset of DC, exhibiting high expression of CD11b and low expression of CD11c, co-stimulator (CD40, CD80, CD83, and CD86) and MHC-II molecules compared to those grown in 2D culture. DCs cultured in the 3D collagen scaffold possessed weak antigen uptake ability and inhibited T-cell proliferation in vitro; in addition, they exhibited potent immunoregulatory function to alleviate allo-delay type hypersensitivity when transferred in vivo. Thus, DCs differentiated in the 3D collagen scaffold were defined as regulatory DCs, indicating that collagen scaffold microenvironments probably play an important role in modulating the lineage commitment of DCs and therefore might be applied as a promising tool for generation of specialised DCs.

引用

页数：12

共 48 条

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