A selective differential medium for Lactobacillus plantarum

被引：96

作者：

Bujalance, Carmen ^{[1
]}

Jimenez-Valera, Maria ^{[1
]}

Moreno, Encarnacion ^{[1
]}

Ruiz-Bravo, Alfonso ^{[1
]}

机构：

[1] Univ Granada, Fac Pharm, Dept Microbiol, E-18071 Granada, Spain

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2006年 / 66卷 / 03期

关键词：

ciprofloxacin; intestinal microbiota; Lactobacillus plantarum; probiotic bacteria; selective medium;

D O I：

10.1016/j.mimet.2006.02.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The quantification of exogenous lactobacilli in faecal samples is frequently required for the evaluation of the intestinal colonization by probiotic bacteria. In this study, a selective and differential medium, designated LPSM, was developed for the culture of exogenous Lactobacillusplantarum. In quantitative assays, LPSM showed a sensitivity similar to those of enriched and Lactobacillus-adapted media. The presence of ciprofloxacin made LPSM inhibitory to most intestinal bacteria, including endogenous acid lactic bacteria, whereas exogenous L. plantarum strains grew producing a yellow color caused by acid production from sorbitol in the presence of bromocresol purple. The results showed that LPSM is suitable for detection and enumeration of L. plantarum in faecal samples. (c) 2006 Elsevier B.V All rights reserved.

引用

页码：572 / 575

页数：4

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