Carbon flux analysis in a pantothenate overproducing Corynebacterium glutamicum strain.

被引:13
作者
Chassagnole, C
Létisse, F
Diano, A
Lindley, ND
机构
[1] Inst Natl Sci Appl, Dept Genie Biochim, UMR 5504, Lab Biotechnol Bioprocedes, F-31077 Toulouse, France
[2] Inst Natl Sci Appl, INRA, UMR 792, Ctr Bioingn Gilbert Durand, F-31077 Toulouse, France
[3] CRITT Bioind, F-31077 Toulouse, France
关键词
Corynebacterium glutamicum; fermentation; flux analysis; metabolic engineering; pantothenate;
D O I
10.1023/A:1020353124066
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Carbon flux analysis during a pseudo-stationary phase of metabolite accumulation in a genetically engineered strain of Corynebacterium glutamicum, containing plasmids leading to over-expression of the ilvBNCD and panBC operons, has identified the basic metabolic constraints governing the potential of this bacterium to produce pantothenate. Carbon flux converging on pyruvate (75% of glucose uptake) is controlled by anabolic precursor requirements and NADPH demand provoking high carbon loss as CO2 via the pentose pathway. Virtually all the flux of pyruvate is directed into the branched pathway leading to both valine and pantothenate production, but flux towards valine is tenfold higher than that transformed to pantothenate, indicating that significant improvements will only be obtained if carbon flux at the ketoisovalerate branchpoint can be modulated.
引用
收藏
页码:129 / 134
页数:6
相关论文
共 13 条
[1]   HIGH-SENSITIVITY AMINO-ACID ANALYSIS BY DERIVATIZATION WITH ORTHO-PHTHALADEHYDE AND 9-FLUORENYLMETHYL CHLOROFORMATE USING FLUORESCENCE DETECTION - APPLICATIONS IN PROTEIN-STRUCTURE DETERMINATION [J].
BLANKENSHIP, DT ;
KRIVANEK, MA ;
ACKERMANN, BL ;
CARDIN, AD .
ANALYTICAL BIOCHEMISTRY, 1989, 178 (02) :227-232
[2]   Growth rate-dependent modulation of carbon flux through central metabolism and the kinetic consequences for glucose-limited chemostat cultures of Corynebacterium glutamicum [J].
CocaignBousquet, M ;
Guyonvarch, A ;
Lindley, ND .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (02) :429-436
[3]  
Dusch N, 1999, APPL ENVIRON MICROB, V65, P1530
[4]   Eco Cyc:: Encyclopedia of Escherichia coli genes and metabolism [J].
Karp, PD ;
Riley, M ;
Paley, SM ;
Pellegrini-Toole, A ;
Krummenacker, M .
NUCLEIC ACIDS RESEARCH, 1999, 27 (01) :55-58
[5]  
Marx A, 1996, BIOTECHNOL BIOENG, V49, P111, DOI 10.1002/(SICI)1097-0290(19960120)49:2<111::AID-BIT1>3.0.CO
[6]  
2-T
[7]  
Nielsen J, 1998, BIOTECHNOL BIOENG, V58, P125, DOI 10.1002/(SICI)1097-0290(19980420)58:2/3<125::AID-BIT3>3.0.CO
[8]  
2-N
[9]  
SAHM H, 1995, FEMS MICROBIOL REV, V16, P243
[10]  
Sahm H, 1999, APPL ENVIRON MICROB, V65, P1973