Stable Isotope Fractionation of γ-Hexachlorocyclohexane (Lindane) during Reductive Dechlorination by Two Strains of Sulfate-Reducing Bacteria

被引：76

作者：

Badea, Silviu-Laurentiu ^{[1
,2
,3
]}

Vogt, Carsten ^{[1
]}

Weber, Stefanie ^{[1
,4
]}

Danet, Andreai-Florin ^{[2
]}

Richnow, Hans-Hermann ^{[1
]}

机构：

[1] UFZ Helmholtz Ctr Environm Res, Dept Isotope Biogeochem, D-04318 Leipzig, Germany

[2] Univ Bucharest, Fac Chem, Bucharest 050657, Romania

[3] NIPNE, Bucharest, Romania

[4] OU Analyt RCC Ltd, CH-4452 Itingen, Switzerland

来源：

ENVIRONMENTAL SCIENCE & TECHNOLOGY | 2009年 / 43卷 / 09期

关键词：

CARBON; DEGRADATION; BIODEGRADATION; BENZENE; DEHALOGENATION; TETRACHLOROETHENE; TRANSFORMATION; HYDROCARBONS; PERSISTENCE; MECHANISMS;

D O I：

10.1021/es801284m

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Carbon isotope fractionation factors were determined with the dichloro elimination of gamma-hexachlorocyclohexane (gamma-HCH) by the sulfate-reducing bacteria Desulfococcus multivorans DSM 2059 and Desulfovibrio, gigas DSM 1382. Both strains are known for cometabolic HCH dechlorination. Degradation experiments with gamma-HCH in concentrations of 22-25 mu M were carried out using benzoate (for D. multivorans) and lactate (for D. gigas) as electron donors, respectively. gamma-HCH was dechlorinated by both bacterial strains within four weeks, and the metabolites gamma-3,4,5,6-tetrachlorocyclohexene (gamma-TCCH), chlorobenzene (CB), and benzene were formed. The carbon isotope fractionation of gamma-HCH dechlorination was quantified by the Rayleigh model, using a bulk enrichment factor (epsilon C) of -3.9 +/- 0.6 for D. gigas and -3.4 +/- 0.5 for D. multivorans, which correspond to apparent kinetic isotope effect (AKIE(c)) values of 1.023 +/- 0.004 or 1.02 +/- 0.003 for stepwise Cl-C bond cleavage. The extent and range of isotope fractionation suggest that gamma-HCH dechlorination can be monitored in anoxic environments by compound-specific isotope analysis (CSIA).

引用

页码：3155 / 3161

页数：7

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