Kinetics of chromium(V) formation and reduction in fronds of the duckweed Spirodela polyrhiza -: a low frequency EPR study

被引:64
作者
Appenroth, KJ
Bischoff, M
Gabrys, H
Stoeckel, J
Swartz, HM
Walczak, T
Winnefeld, K
机构
[1] Jagiellonian Univ, Inst Mol Biol, PL-31120 Krakow, Poland
[2] Univ Jena, Dept Plant Physiol, Inst Gen Bot, D-6900 Jena, Germany
[3] Univ Jena, Inst Opt & Quantum Elect, D-6900 Jena, Germany
[4] Dartmouth Med Sch, Dept Radiol, Hanover, NH 03755 USA
[5] Univ Jena, Inst Clin Chem & Lab Diagnost, D-6900 Jena, Germany
关键词
chromium(V); duckweed Spirodela polyrhiza; EPR study;
D O I
10.1016/S0162-0134(00)00018-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The uptake of chromate by the duckweed Spirodela polyrhiza was investigated with atomic absorption spectroscopy and the reduction of Cr( VI) to Cr(V) was measured using low frequency EPR spectroscopy. The biphasic kinetics of the uptake was fitted to parameters of a proposed kinetic model. Another model was developed to simulate chromate reduction. The first step of chromate reduction was found to be much faster than the uptake of Cr(VI) from the free space. Most probably, this step occurs already in the cell wall or on the cell membrane surface. Further reduction of Cr(V) to Cr(III) was estimated to be slower. The disappearance of the Cr(V) signal, following transfer of the plants into a Cr-free solution, lasted several tens of hours; the kinetics was mono- or biexponential depending on the length of Cr loading. The rate constants for Cr reduction in living plants were determined for the first time. (C) 2000 Elsevier Science Inc. All rights reserved.
引用
收藏
页码:235 / 242
页数:8
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