A high-performance liquid-chromatographic method for the determination of ochratoxin A in human plasma

被引：15

作者：

Jimenez, AM

de Cerain, AL ^{[1
]}

Gonzalez-Peñas, E

Bello, J

机构：

[1] Univ Navarra, Dept Food Sci & Technol, Fac Pharm, E-31080 Pamplona, Spain

[2] Univ Navarra, Dept Organ Pharmaceut Chem, Fac Pharm, E-31080 Pamplona, Spain

来源：

CHROMATOGRAPHIA | 1999年 / 50卷 / 7-8期

关键词：

column liquid chromatography; ochratoxin A; mycotoxins; validation;

D O I：

10.1007/BF02490742

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance liquid-chromatographic method is described for the quantitative determination of the mycotoxin ochratoxin A (OTA) in human plasma. The assay involves extraction with chloroform and sodium bicarbonate then HPLC with fluorescence detection. The method was validated in terms of selectivity, recovery, linearity, precision (within-day and between-day variability), accuracy, detection and quantification limits, and the stability of OTA in plasma and treated samples. The limit of detection was 0.4 ng mL(-1) of OTA in methanol, corresponding to 0.52 ng ml(-1) OTA in plasma. This assay was successfully applied for the determination of OTA levels in human plasma.

引用

页码：457 / 460

页数：4

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