VDAC channels mediate and gate the flow of ATP: Implications for the regulation of mitochondrial function

被引:292
作者
Rostovtseva, T [1 ]
Colombini, M [1 ]
机构
[1] UNIV MARYLAND,DEPT ZOOL,CELL BIOL LAB,COLLEGE PK,MD 20742
关键词
D O I
10.1016/S0006-3495(97)78841-6
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
The mitochondrial channel, VDAC, forms large (3 nm in diameter) aqueous pores through membranes. We measured ATP flow (using the luciferin/luciferase method) through these channels after reconstitution into planar phospholipid membranes. In the open state of VDAC, as many as 2 x 10(6) ATP molecules can flow through one channel per second. The half-maximum rate occurs at similar to 75 mM ATP. The permeability of a single channel for ATP is 1.1 x 10(-14) cm(3)/s (about 1 cm/s after correcting for cross-sectional area), which is 100 times less than the permeability for chloride and 10 times less than that for succinate. Channel closure results in a 50% reduction in conductance, showing that monovalent ions are still quite permeable, yet ATP flux is almost totally blocked. This is consistent with an electrostatic barrier that results in inversion of the selectivity of the channel and could be an example of how large channels selectively control the flow of charged metabolites. Thus VDAC is ideally suited to controlling the flow of ATP between the cytosol and the mitochondrial spaces.
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页码:1954 / 1962
页数:9
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