Detection by polymerase chain reaction of Schistosoma mansoni DNA in human serum and feces

被引：153

作者：

Pontes, LA ^{[1
]}

Dias-Neto, E ^{[1
]}

Rabello, A ^{[1
]}

机构：

[1] Ludwig Inst Canc Res, Canc Genet Lab, BR-01509010 Sao Paulo, Brazil

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2002年 / 66卷 / 02期

关键词：

D O I：

10.4269/ajtmh.2002.66.157

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

A novel method for the detection of Schistosoma mansoni in human samples that is based on the amplification of a highly repeated DNA sequence has been developed. By use of simple DNA extraction techniques and a rapid 2-step polymerase chain reaction (PCR). it was possible to amplify S. mansoni DNA in human fecal and serum samples. The high sensitivity of the approach enabled the detection of the parasite DNA in fecal samples containing as few as 2,4 eggs per gram of feces, which makes it 10 times more sensitive than the Kato-Katz examination. A detection limit of 1 fg of Schistosoma sp. DNA was determined when pure DNA was used as PCR template. The amplification reaction showed to be specific giving no cross-reaction with DNA from other helminths. The PCR assay developed in this study may constitute a valuable alternative for the diagnosis of the Schistosoma sp. infection.

引用

页码：157 / 162

页数：6

共 24 条

[1] Application of immunodiagnostic assays: Detection of antibodies and circulating antigens in human schistosomiasis and correlation with clinical findings [J].

Al-Sherbiny, MM ;

Osman, AM ;

Hancock, K ;

Deelder, AM ;

Tsang, VCW .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1999, 60 (06) :960-966

[2] A SIMPLE METHOD TO DETECT PLASMODIUM-FALCIPARUM DIRECTLY FROM BLOOD-SAMPLES USING THE POLYMERASE CHAIN-REACTION [J].

BARKER, RH ;

BANCHONGAKSORN, T ;

COURVAL, JM ;

SUWONKERD, W ;

RIMWUNGTRAGOON, K ;

WIRTH, DF .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1992, 46 (04) :416-426

[3] DIAGNOSIS OF HUMAN SCHISTOSOMIASIS BY DETECTION OF CIRCULATING CATHODIC ANTIGEN WITH A MONOCLONAL-ANTIBODY [J].

BARSOUM, IS ;

KAMAL, KA ;

BASSILY, S ;

DEELDER, AM ;

COLLEY, DG .

JOURNAL OF INFECTIOUS DISEASES, 1991, 164 (05) :1010-1013

[4] LEVELS OF THE SCHISTOSOME CIRCULATING ANODIC AND CATHODIC ANTIGENS IN SERUM OF SCHISTOSOMIASIS PATIENTS FROM BRAZIL [J].

DEJONGE, N ;

RABELLO, ALT ;

KRIJGER, FW ;

KREMSNER, PG ;

ROCHA, RS ;

KATZ, N ;

DEELDER, AM .

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1991, 85 (06) :756-759

[5] AN IMPROVED POLYMERASE CHAIN-REACTION ASSAY TO DETECT TRYPANOSOMA-CRUZI IN BLOOD [J].

DIAZ, C ;

NUSSENZWEIG, V ;

GONZALEZ, A .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1992, 46 (05) :616-623

[6] SEROEPIDEMIOLOGY AND SERODIAGNOSIS OF SCHISTOSOMIASIS IN KENYA USING CRUDE AND PURIFIED EGG ANTIGENS OF SCHISTOSOMA-MANSONI IN ELISA [J].

DOENHOFF, MJ ;

BUTTERWORTH, AE ;

HAYES, RJ ;

STURROCK, RF ;

OUMA, JH ;

KOECH, D ;

PRENTICE, M ;

BAIN, J .

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1993, 87 (01) :42-48

[7] CLONING OF A 21.7-KDA VACCINE-DOMINANT ANTIGEN GENE OF SCHISTOSOMA-MANSONI REVEALS AN EF HAND-LIKE MOTIF [J].

FRANCIS, P ;

BICKLE, Q .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1992, 50 (02) :215-224

[8] SEXING SINGLE LARVAL STAGES OF SCHISTOSOMA-MANSONI BY POLYMERASE CHAIN-REACTION [J].

GASSER, RB ;

MORAHAN, G ;

MITCHELL, GF .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1991, 47 (02) :255-258

[9] Development and laboratory evaluation of a polymerase chain reaction for monitoring Schistosoma mansoni infestation of water [J].

Hamburger, J ;

Xu, YX ;

Ramzy, RM ;

Jourdane, J ;

Ruppel, A .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1998, 59 (03) :468-473

[10] HIGHLY REPEATED SHORT DNA-SEQUENCES IN THE GENOME OF SCHISTOSOMA-MANSONI RECOGNIZED BY A SPECIES-SPECIFIC PROBE [J].

HAMBURGER, J ;

TURETSKI, T ;

KAPELLER, I ;

DERESIEWICZ, R .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1991, 44 (01) :73-80

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