H+-Pyrophosphatase of Rhodospirillum rubrum -: High yield expression in Escherichia coli and identification of the Cys residues responsible for inactivation by mersalyl

被引:31
作者
Belogurov, GA
Turkina, MV
Penttinen, A
Huopalahti, S
Baykov, AA [1 ]
Lahti, R
机构
[1] Univ Turku, Dept Biochem & Food Chem, FIN-20014 Turku, Finland
[2] Moscow MV Lomonosov State Univ, AN Belozersky Inst Physicochem Biol, Moscow 119899, Russia
关键词
D O I
10.1074/jbc.M202951200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
H+-translocating pyrophosphatase (H+-PPase) of the photosynthetic bacterium Rhodospirillum rubrum was expressed in Escherichia coli C43(DE3) cells. Recombinant H+-PPase was observed in inner membrane vesicles, where it catalyzed both PPi hydrolysis coupled with H+ transport into the vesicles and PPi synthesis. The hydrolytic activity of H+-PPase in E. coli vesicles was eight times greater than that in R. rubrum chromatophores but exhibited similar sensitivity to the H+-PPase inhibitor, aminomethylenediphosphonate, and insensitivity to the soluble PPase inhibitor, fluoride. Using this expression system, we showed that substitution of Cys(185), Cys(222), or Cys(573) with aliphatic residues had no effect on the activity of H+-PPase but decreased its sensitivity to the sulfhydryl modifying reagent, mersalyl. H+-PPase lacking all three Cys residues was completely resistant to the effects of mersalyl. Mg2+ and MgPPi protected Cys(185) and Cys(573) from modification by this agent but not Cys(222) Phylogenetic analyses of 23 nonredundant H+-PPase sequences led to classification into two subfamilies. One subfamily invariably contains Cys(222) and includes all known K+-independent H+-PPases, whereas the other incorporates a conserved Cys(573) but lacks Cys(222) and includes all known K+-dependent H+-PPases. These data suggest a specific link between the incidence of Cys at positions 222 and 573 and the K+ dependence of H+-PPase.
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页码:22209 / 22214
页数:6
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