Regulation of dendritic protein synthesis by miniature synaptic events

被引:203
作者
Sutton, MA [1 ]
Wall, NR [1 ]
Aakalu, GN [1 ]
Schuman, EM [1 ]
机构
[1] CALTECH, Howard Hughes Med Inst, Div Biol, Pasadena, CA 91125 USA
关键词
D O I
10.1126/science.1096202
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We examined dendritic protein synthesis after a prolonged blockade of action potentials alone and after a blockade of both action potentials and miniature excitatory synaptic events ( minis). Relative to controls, dendrites exposed to a prolonged blockade of action potentials showed diminished protein synthesis. Dendrites in which both action potentials and minis were blocked showed enhanced protein synthesis, suggesting that minis inhibit dendritic translation. When minis were acutely blocked or stimulated, an immediate increase or decrease, respectively, in dendritic translation was observed. Taken together, these results reveal a role for miniature synaptic events in the acute regulation of dendritic protein synthesis in neurons.
引用
收藏
页码:1979 / 1983
页数:5
相关论文
共 29 条
[1]   Dynamic visualization of local protein synthesis in hippocampal neurons [J].
Aakalu, G ;
Smith, WB ;
Nguyen, N ;
Jiang, CG ;
Schuman, EM .
NEURON, 2001, 30 (02) :489-502
[2]   α-latrotoxin, acting via two Ca2+-dependent pathways, triggers exocytosis of two pools of synaptic vesicles [J].
Ashton, AC ;
Volynski, KE ;
Lelianova, VG ;
Orlova, EV ;
Van Renterghem, C ;
Canepari, M ;
Seagar, M ;
Ushkaryov, YA .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (48) :44695-44703
[3]   Multiple forms of synaptic plasticity triggered by selective suppression of activity in individual neurons [J].
Burrone, J ;
O'Byrne, M ;
Murthy, VN .
NATURE, 2002, 420 (6914) :414-418
[4]   A transient, neuron-wide form of CREB-mediated long-term facilitation can be stabilized at specific synapses by local protein synthesis [J].
Casadio, A ;
Martin, KC ;
Giustetto, M ;
Zhu, HX ;
Chen, M ;
Bartsch, D ;
Bailey, CH ;
Kandel, ER .
CELL, 1999, 99 (02) :221-237
[5]   TURNOVER OF TRANSMITTER AND SYNAPTIC VESICLES AT FROG NEUROMUSCULAR JUNCTION [J].
CECCARELLI, B ;
HURLBUT, WP ;
MAURO, A .
JOURNAL OF CELL BIOLOGY, 1973, 57 (02) :499-524
[6]   Synapse-specific control of synaptic efficacy at the terminals of a single neuron [J].
Davis, GW ;
Goodman, CS .
NATURE, 1998, 392 (6671) :82-86
[7]   An activity-dependent switch to cap-independent translation triggered by eIF4E dephosphorylation [J].
Dyer, JR ;
Michel, S ;
Lee, W ;
Castellucci, VF ;
Wayne, NL ;
Sossin, WS .
NATURE NEUROSCIENCE, 2003, 6 (03) :219-220
[8]  
FATT P, 1952, J PHYSIOL-LONDON, V117, P109
[10]   Role for rapid dendritic protein synthesis in hippocampal mGluR-dependent long-term depression [J].
Huber, KM ;
Kayser, MS ;
Bear, MF .
SCIENCE, 2000, 288 (5469) :1254-1256