Markers of coagulation, fibrinolysis and angiogenesis after strenuous short-term exercise (Wingate-test) in male subjects of varying fitness levels

It was the aim of the study to analyse the haemostatic system during a high standardized intensive short-term (30 s) exercise (anaerobic Wingate test). Blood samples were taken from 15 male subjects before (to), and within 2 (t(1)), 9 and 30 min (t(3)) after the test. We found that the partial thromboplastin time was markedly shortened, whereas the prothrombin time increased slightly from to to t(1), (p < 0.002) and remained elevated %, (p < 0.046). Factor VIII increased from to to t(1), (p < 0.001) and remained elevated as well %, (p < 0.001). Fibrin monomers were approximately 15 times higher immediately post-exercise (t(1), p < 0.001) and continued to be elevated (t(3), p < 0.004). The tissue plasminogen activator increased by 4 times after exercise (t(1), p < 0.001) and remained elevated %, (p < 0.002). The d-dimers increased from to to t(1), (p < 0.001) as well and remained elevated (t(3), p < 0.005). Thrombopoietin concentrations were unchanged, whereas the vascular endothelial growth factor increased immediately post-exercise (to to t(1), p < 0.011 resp. at t(2) p < 0.019) and returned to the control level at t(3) (p < 0.878). In conclusion, it was found that prothrombotic markers and, even more pronounced, those of the fibrinolytic system were increased. The study provides evidence that due to intensive short-term exercise the balance of the haemostatic system is shifted to a higher equilibrium. Theoretically, the data show that in the case of a subject with risk factors such as impaired fibrinolysis, unfavourable conditions cannot be excluded.