Anti-estrogen ICI 182.780 and anti-androgen flutamide induce tyrosine phosphorylation of cortactin in the ectoplasmic specialization between the Sertoli cell and spermatids in the mouse testis

被引：9

作者：

Anahara, R

Toyama, Y

Maekawa, M

Yoshida, M

Kai, M

Ishino, F

Toshimori, K

Mori, C

机构：

[1] Chiba Univ, Grad Sch Med, Dept Bioenvironm Med, Chuo Ku, Chiba 2608670, Japan

[2] Chiba Univ, Grad Sch Med, Dept Anat & Dev Biol, Chuo Ku, Chiba 2608670, Japan

[3] Ryotokuji Univ, Fac Hlth Sci, Dept Phys Therapy, Chiba 2798567, Japan

[4] Tokyo Inst Technol, Ctr Biol Resources & Informat, Div Gene Res, Midori Ku, Yokohama, Kanagawa 2268501, Japan

[5] Tokyo Med & Dent Univ, Med Res Inst, Chiyoda Ku, Tokyo 1010062, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2006年 / 346卷 / 01期

关键词：

cortactin; ectoplasmic specialization; tyrosine phosphorylation; depolymerization; ICI; 182.780; flutamide;

D O I：

10.1016/j.bbrc.2006.05.125

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Our previous study revealed that the ectoplasmic specialization (ES) was deleted by the treatment of anti-estrogen, ICI 182.780 (ICI), and anti-androgen, flutamide (FLUT) in mouse testis. Also, expression of cortactin, an F-actin-binding protein, was decreased by the treatment of FLUT in mouse testis. Cortactin has been suggested to promote actin polymerizer at the ES in the testis, and also actin depolymerization is induced by tyrosine phosphorylation of cortactin. The present study revealed that exogenous treatment of ICI and FLUT caused the deletion of the cortactin in the apical ES and the increase of tyrosine phosphorylated cortactin in mouse testis. These results suggest that the sex hormone antagonists', ICI and FLUT, induced actin depolymerization and tyrosine phosphorylation of cortactin in the mouse testis. Also, the present study may be a key to elucidate the adverse affect exogenous compounds that affect spermiation. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：276 / 280

页数：5

共 22 条

[1]

Flutamide induces ultrastructural changes in spermatids and the ectoplasmic specialization between the Sertoli cell and spermatids in mouse testes [J].