Flavonoids inhibit the amidolytic activity of human thrombin

被引：55

作者：

Mozzicafreddo, M. ^{[1
]}

Cuccioloni, M. ^{[1
]}

Eleuteri, A. M. ^{[1
]}

Fioretti, E. ^{[1
]}

Angeletti, M. ^{[1
]}

机构：

[1] Univ Camerino, MCAB Dept, I-62032 Camerino, MC, Italy

来源：

BIOCHIMIE | 2006年 / 88卷 / 09期

关键词：

bioinformatics; biosensor; equilibrium studies; kinetics; polyphenols;

D O I：

10.1016/j.biochi.2006.04.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The effect of a group of natural flavonoids on human thrombin amidolytic activity was investigated using a spectrophotometric inhibition assay while information on the kinetics and thermodynamics was obtained using optical biosensor techniques. All the flavonoids tested acted as reversible inhibitors, and the quercetin-thrombin complex was found to be most stable at pH = 7.5. Docking analysis indicated that quercetin's inhibitory behavior could be related to its planar structure and low steric hindrance, and to its ability to form a critical H-bond with thrombin His57. (c) 2006 Elsevier SAS. All rights reserved.

引用

页码：1297 / 1306

页数：10

共 91 条

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Biochemical responses of chestnut oak to a galling cynipid [J].