A caspase-related protease regulates apoptosis in yeast

被引:733
作者
Madeo, F
Herker, E
Maldener, C
Wissing, S
Lächelt, S
Herian, M
Fehr, M
Lauber, K
Sigrist, SJ
Wesselborg, S
Fröhlich, KU
机构
[1] Univ Tubingen, Inst Physiol Chem, D-72076 Tubingen, Germany
[2] Adolf Butenandt Inst Physiol Chem, D-80336 Munich, Germany
[3] Univ Tubingen, Ctr Mol Biol Plants, D-72076 Tubingen, Germany
[4] Univ Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany
[5] European Neurosci Inst Gottingen, D-37073 Gottingen, Germany
[6] Karl Franzens Univ Graz, IMBM, A-8010 Graz, Austria
关键词
D O I
10.1016/S1097-2765(02)00501-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Yeast can undergo cell death accompanied by cellular markers of apoptosis. However, orthologs of classical mammalian apoptosis regulators appeared to be missing from the yeast genome, challenging a common mechanism of yeast and mammalian apoptosis. Here we investigate Yor197w, a yeast protein with structural homology to mammalian caspases, and demonstrate caspase-like processing of the protein. Hydrogen peroxide treatment induces apoptosis together with a caspase-like enzymatic activity in yeast. This response is completely abrogated after disruption and strongly stimulated after overexpression of Yor197w. Yor197w also mediates the death process within chronologically aged cultures, pointing to a physiological role in elimination of overaged cells. We conclude that Yor197w indeed functions as a bona fide caspase in yeast and propose the name Yeast Caspase-1 (YCA1, gene YCA1).
引用
收藏
页码:911 / 917
页数:7
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